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An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads

This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA...

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Main Authors: Lim, Crystale Siew Ying, Tung, Chee Hong, Rosli, Rozita, Chong, Pei Pei
Format: Article
Language:English
Published: Elsevier 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf
http://psasir.upm.edu.my/id/eprint/13008/
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spelling my.upm.eprints.130082017-03-28T14:04:09Z http://psasir.upm.edu.my/id/eprint/13008/ An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads Lim, Crystale Siew Ying Tung, Chee Hong Rosli, Rozita Chong, Pei Pei This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA. Elsevier 2008-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf Lim, Crystale Siew Ying and Tung, Chee Hong and Rosli, Rozita and Chong, Pei Pei (2008) An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads. Journal of Microbiological Methods, 75 (3). pp. 576-578. ISSN 0167-7012; ESSN: 1872-8359 10.1016/j.mimet.2008.07.026
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA.
format Article
author Lim, Crystale Siew Ying
Tung, Chee Hong
Rosli, Rozita
Chong, Pei Pei
spellingShingle Lim, Crystale Siew Ying
Tung, Chee Hong
Rosli, Rozita
Chong, Pei Pei
An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
author_facet Lim, Crystale Siew Ying
Tung, Chee Hong
Rosli, Rozita
Chong, Pei Pei
author_sort Lim, Crystale Siew Ying
title An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
title_short An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
title_full An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
title_fullStr An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
title_full_unstemmed An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
title_sort alternative candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
publisher Elsevier
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf
http://psasir.upm.edu.my/id/eprint/13008/
_version_ 1643825200877273088
score 13.211869

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