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Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line

While dietary components such as phytate reduce calcium absorption, bioactive peptides could potentially enhance calcium absorption. This study aimed to characterise the peptides derived from casein and assess their effect on calcium uptake by Caco-2 cells. Casein was subjected to hydrolysis by Alca...

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Main Authors: Chew, Lye Yee, Toh, Gaik Theng, Ismail, Amin, Shafie, Nurul Husna, Mat Daud, Zulfitri 'Azuan
Format: Article
Language:English
Published: John Wiley and Sons 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115464/1/115464.pdf
http://psasir.upm.edu.my/id/eprint/115464/
https://ifst.onlinelibrary.wiley.com/doi/10.1111/ijfs.17199
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spelling my.upm.eprints.1154642025-03-04T08:22:31Z http://psasir.upm.edu.my/id/eprint/115464/ Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line Chew, Lye Yee Toh, Gaik Theng Ismail, Amin Shafie, Nurul Husna Mat Daud, Zulfitri 'Azuan While dietary components such as phytate reduce calcium absorption, bioactive peptides could potentially enhance calcium absorption. This study aimed to characterise the peptides derived from casein and assess their effect on calcium uptake by Caco-2 cells. Casein was subjected to hydrolysis by Alcalase and Flavourzyme to produce 4-h and 16-h casein hydrolysates. High-performance liquid chromatography analysis showed that hydrolysis caused significant increases and decreases in the content of some amino acids. Casein hydrolysates achieved 40.36% and 52.64% degree of hydrolysis, as determined by the o-phthaldialdehyde method. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and dynamic light scattering analyses revealed that the peptides had molecular weight less than 15 kDa and diameter between 100 and 1000 nm. The 4-h casein hydrolysate enhanced calcium uptake by Caco-2 cells monolayer at low calcium intake, possibly via transcellular absorption. These results provide insight into the nutraceutical potential of casein hydrolysates as enhancer of calcium absorption. John Wiley and Sons 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115464/1/115464.pdf Chew, Lye Yee and Toh, Gaik Theng and Ismail, Amin and Shafie, Nurul Husna and Mat Daud, Zulfitri 'Azuan (2024) Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line. International Journal of Food Science and Technology, 59 (10). pp. 7952-7961. ISSN 0950-5423; eISSN: 1365-2621 https://ifst.onlinelibrary.wiley.com/doi/10.1111/ijfs.17199 10.1111/ijfs.17199
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description While dietary components such as phytate reduce calcium absorption, bioactive peptides could potentially enhance calcium absorption. This study aimed to characterise the peptides derived from casein and assess their effect on calcium uptake by Caco-2 cells. Casein was subjected to hydrolysis by Alcalase and Flavourzyme to produce 4-h and 16-h casein hydrolysates. High-performance liquid chromatography analysis showed that hydrolysis caused significant increases and decreases in the content of some amino acids. Casein hydrolysates achieved 40.36% and 52.64% degree of hydrolysis, as determined by the o-phthaldialdehyde method. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and dynamic light scattering analyses revealed that the peptides had molecular weight less than 15 kDa and diameter between 100 and 1000 nm. The 4-h casein hydrolysate enhanced calcium uptake by Caco-2 cells monolayer at low calcium intake, possibly via transcellular absorption. These results provide insight into the nutraceutical potential of casein hydrolysates as enhancer of calcium absorption.
format Article
author Chew, Lye Yee
Toh, Gaik Theng
Ismail, Amin
Shafie, Nurul Husna
Mat Daud, Zulfitri 'Azuan
spellingShingle Chew, Lye Yee
Toh, Gaik Theng
Ismail, Amin
Shafie, Nurul Husna
Mat Daud, Zulfitri 'Azuan
Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
author_facet Chew, Lye Yee
Toh, Gaik Theng
Ismail, Amin
Shafie, Nurul Husna
Mat Daud, Zulfitri 'Azuan
author_sort Chew, Lye Yee
title Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
title_short Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
title_full Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
title_fullStr Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
title_full_unstemmed Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line
title_sort casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by caco-2 cell line
publisher John Wiley and Sons
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/115464/1/115464.pdf
http://psasir.upm.edu.my/id/eprint/115464/
https://ifst.onlinelibrary.wiley.com/doi/10.1111/ijfs.17199
_version_ 1825810757683511296
score 13.244413

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