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Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles

Luteolin is a widely studied flavonoid recognized for its ability to sensitize multidrug-resistant cells and anti-cancer properties. However, its clinical applications is hindered by challenges arising from its limited solubility and bioavailability. To address this issue, a synergistic approach com...

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Main Authors: Mod Razif, Muhammad Redza Fahmi, Liew, Kai Bin, Chan, Siok Yee, Chew, Yik Ling, Hassan, Masriana, Ahmad Hisham, Shairyzah, Abdul Rahman, Shamima, Kee, Phei Er
Format: Article
Language:English
Published: Association of Biotechnology and Pharmacy 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114388/1/114388.pdf
http://psasir.upm.edu.my/id/eprint/114388/
https://abap.co.in/index.php/home/article/view/1570
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spelling my.upm.eprints.1143882025-01-20T00:50:42Z http://psasir.upm.edu.my/id/eprint/114388/ Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles Mod Razif, Muhammad Redza Fahmi Liew, Kai Bin Chan, Siok Yee Chew, Yik Ling Hassan, Masriana Ahmad Hisham, Shairyzah Abdul Rahman, Shamima Kee, Phei Er Luteolin is a widely studied flavonoid recognized for its ability to sensitize multidrug-resistant cells and anti-cancer properties. However, its clinical applications is hindered by challenges arising from its limited solubility and bioavailability. To address this issue, a synergistic approach combining luteolin with vitamin E TPGS (TPGS) and poloxamer (Pol) was explored to boost tumour apoptosis and suppress P-glycoprotein. This study aimed to optimize luteolin-loaded TPGS/Pol micelles developed using the film hydration method, followed by lyophilization. Various key factors including the drug: polymer ratio and the TPGS: Pol ratio were examined, with encapsulation efficiency (EE) assessed using a UV-Vis spectrophotometer and particle size measured via dynamic light scattering (DLS). The results revealed that the optimized micelle composed of 1:5 drug: polymer ratio and a TPGS: Pol ratio of 3:1, exhibited a particle size below 40 nm, along with EE of 90%. Additionally, there was a notable increase of 459-fold in the solubility of luteolin-loaded micelle in comparison to pure luteolin in water. The TPGS/Pol micelles demonstrated a critical micelle concentration (CMC) of 0.0008 mg/ml, and release studies indicated sustained release behaviour for luteolin-loaded micelles. In conclusion, this study has presented the feasibility of TPGS/Pol micelles in improving the therapeutic potential of luteolin, showcasing improvements in EE, particle size, solubility and sustained release behaviour. Association of Biotechnology and Pharmacy 2024-09 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/114388/1/114388.pdf Mod Razif, Muhammad Redza Fahmi and Liew, Kai Bin and Chan, Siok Yee and Chew, Yik Ling and Hassan, Masriana and Ahmad Hisham, Shairyzah and Abdul Rahman, Shamima and Kee, Phei Er (2024) Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles. Current Trends in Biotechnology and Pharmacy, 18 (3s). pp. 209-218. ISSN 0973-8916; eISSN: 2230-7303 https://abap.co.in/index.php/home/article/view/1570 10.5530/ctbp.2024.3s.16
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Luteolin is a widely studied flavonoid recognized for its ability to sensitize multidrug-resistant cells and anti-cancer properties. However, its clinical applications is hindered by challenges arising from its limited solubility and bioavailability. To address this issue, a synergistic approach combining luteolin with vitamin E TPGS (TPGS) and poloxamer (Pol) was explored to boost tumour apoptosis and suppress P-glycoprotein. This study aimed to optimize luteolin-loaded TPGS/Pol micelles developed using the film hydration method, followed by lyophilization. Various key factors including the drug: polymer ratio and the TPGS: Pol ratio were examined, with encapsulation efficiency (EE) assessed using a UV-Vis spectrophotometer and particle size measured via dynamic light scattering (DLS). The results revealed that the optimized micelle composed of 1:5 drug: polymer ratio and a TPGS: Pol ratio of 3:1, exhibited a particle size below 40 nm, along with EE of 90%. Additionally, there was a notable increase of 459-fold in the solubility of luteolin-loaded micelle in comparison to pure luteolin in water. The TPGS/Pol micelles demonstrated a critical micelle concentration (CMC) of 0.0008 mg/ml, and release studies indicated sustained release behaviour for luteolin-loaded micelles. In conclusion, this study has presented the feasibility of TPGS/Pol micelles in improving the therapeutic potential of luteolin, showcasing improvements in EE, particle size, solubility and sustained release behaviour.
format Article
author Mod Razif, Muhammad Redza Fahmi
Liew, Kai Bin
Chan, Siok Yee
Chew, Yik Ling
Hassan, Masriana
Ahmad Hisham, Shairyzah
Abdul Rahman, Shamima
Kee, Phei Er
spellingShingle Mod Razif, Muhammad Redza Fahmi
Liew, Kai Bin
Chan, Siok Yee
Chew, Yik Ling
Hassan, Masriana
Ahmad Hisham, Shairyzah
Abdul Rahman, Shamima
Kee, Phei Er
Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
author_facet Mod Razif, Muhammad Redza Fahmi
Liew, Kai Bin
Chan, Siok Yee
Chew, Yik Ling
Hassan, Masriana
Ahmad Hisham, Shairyzah
Abdul Rahman, Shamima
Kee, Phei Er
author_sort Mod Razif, Muhammad Redza Fahmi
title Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
title_short Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
title_full Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
title_fullStr Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
title_full_unstemmed Enhancing breast cancer treatment: investigating the influence of polymer ratios on Luteolin-loaded TPGS/poloxamer micelles
title_sort enhancing breast cancer treatment: investigating the influence of polymer ratios on luteolin-loaded tpgs/poloxamer micelles
publisher Association of Biotechnology and Pharmacy
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114388/1/114388.pdf
http://psasir.upm.edu.my/id/eprint/114388/
https://abap.co.in/index.php/home/article/view/1570
_version_ 1823093141247361024
score 13.244109

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