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Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions

Palm stearin and palm kernel olein at 1:1 (w/w) ratio was transesterified using 1.0% w/w lipase from Rhizomucor miehei (Lipozyme IM60, Novo Nordisk). The transesterified blend, as well as its isopropanol-solid (fat solid fractionated using isopropanol at 5 °C) and high melting glycerides (HMG, fat s...

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Main Authors: Chu, B.S., Ghazali, H.M., Lai, O.M., Che Man, Y.B., Yusof, S.
Format: Article
Language:English
Published: Elsevier 2002
Online Access:http://psasir.upm.edu.my/id/eprint/112933/1/112933.pdf
http://psasir.upm.edu.my/id/eprint/112933/
https://linkinghub.elsevier.com/retrieve/pii/S0308814601002564
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spelling my.upm.eprints.1129332025-01-27T06:59:20Z http://psasir.upm.edu.my/id/eprint/112933/ Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions Chu, B.S. Ghazali, H.M. Lai, O.M. Che Man, Y.B. Yusof, S. Palm stearin and palm kernel olein at 1:1 (w/w) ratio was transesterified using 1.0% w/w lipase from Rhizomucor miehei (Lipozyme IM60, Novo Nordisk). The transesterified blend, as well as its isopropanol-solid (fat solid fractionated using isopropanol at 5 °C) and high melting glycerides (HMG, fat solid fractionated using acetone at 10 °C) were studied. Low carbon number triacylglycerols (TG), ranging from C32 to C38, and high carbon number TG (C48 and C50) were used to produce TG ranging from C40 to C46. Transesterification did not much alter the fatty acid (FA) composition of the mixture, but it increased the level of C12:0 (lauric acid) and reduced the C16:0 (palmitic acid) level in the isopropanol-solid and HMG fractions compared to the control. Transesterification also did not much alter the polymorphic forms of the mixture. However, the HMG fraction of the transesterified mixture had more tendency to form β' crystals than the control. Slip melting point of transesterified fat was lower than the control. Heating thermograms of differential scanning calorimetry (DSC) illustrated that the high-melting TG peaks in the transesterified blend were reduced in size while the low-melting TG peaks were broadened, mainly due to the rearrangement of FA to form lower-melting TG. The temperature of the high-melting TG peaks of all samples showed a good correlation (correlation coefficient, r=0.9899; P<0.05) with PPP (P, palmitic acid) levels. Formations of low-melting TG also broadened the low-temperature (low-T) peak in the cooling curve of DSC and reduced the crystallisation temperature of the low-T peak. The high-temperature (high-T) peak of the cooling curves were more dominant in the isopropanol-solid and HMG fractions of both transesterified and control blends. The high-T peaks of the transesterified blend was broader and had a lower temperature than the control. Transesterified blends had lower solid fat contents (SFC) than the control at all temperatures. Production of more low-melting TG in the transesterified blend caused a sharp drop in SFC of the control which shifted from the range of 15-20 °C to 10-15 °C. Elsevier 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112933/1/112933.pdf Chu, B.S. and Ghazali, H.M. and Lai, O.M. and Che Man, Y.B. and Yusof, S. (2002) Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions. Food Chemistry, 76 (2). pp. 155-164. ISSN 0308-8146; eISSN: 1873-7072 https://linkinghub.elsevier.com/retrieve/pii/S0308814601002564 10.1016/s0308-8146(01)00256-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Palm stearin and palm kernel olein at 1:1 (w/w) ratio was transesterified using 1.0% w/w lipase from Rhizomucor miehei (Lipozyme IM60, Novo Nordisk). The transesterified blend, as well as its isopropanol-solid (fat solid fractionated using isopropanol at 5 °C) and high melting glycerides (HMG, fat solid fractionated using acetone at 10 °C) were studied. Low carbon number triacylglycerols (TG), ranging from C32 to C38, and high carbon number TG (C48 and C50) were used to produce TG ranging from C40 to C46. Transesterification did not much alter the fatty acid (FA) composition of the mixture, but it increased the level of C12:0 (lauric acid) and reduced the C16:0 (palmitic acid) level in the isopropanol-solid and HMG fractions compared to the control. Transesterification also did not much alter the polymorphic forms of the mixture. However, the HMG fraction of the transesterified mixture had more tendency to form β' crystals than the control. Slip melting point of transesterified fat was lower than the control. Heating thermograms of differential scanning calorimetry (DSC) illustrated that the high-melting TG peaks in the transesterified blend were reduced in size while the low-melting TG peaks were broadened, mainly due to the rearrangement of FA to form lower-melting TG. The temperature of the high-melting TG peaks of all samples showed a good correlation (correlation coefficient, r=0.9899; P<0.05) with PPP (P, palmitic acid) levels. Formations of low-melting TG also broadened the low-temperature (low-T) peak in the cooling curve of DSC and reduced the crystallisation temperature of the low-T peak. The high-temperature (high-T) peak of the cooling curves were more dominant in the isopropanol-solid and HMG fractions of both transesterified and control blends. The high-T peaks of the transesterified blend was broader and had a lower temperature than the control. Transesterified blends had lower solid fat contents (SFC) than the control at all temperatures. Production of more low-melting TG in the transesterified blend caused a sharp drop in SFC of the control which shifted from the range of 15-20 °C to 10-15 °C.
format Article
author Chu, B.S.
Ghazali, H.M.
Lai, O.M.
Che Man, Y.B.
Yusof, S.
spellingShingle Chu, B.S.
Ghazali, H.M.
Lai, O.M.
Che Man, Y.B.
Yusof, S.
Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
author_facet Chu, B.S.
Ghazali, H.M.
Lai, O.M.
Che Man, Y.B.
Yusof, S.
author_sort Chu, B.S.
title Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
title_short Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
title_full Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
title_fullStr Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
title_full_unstemmed Physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
title_sort physical and chemical properties of a lipase-transesterified palm stearin/palm kernel olein blend and its isopropanol-solid and high melting triacylglycerol fractions
publisher Elsevier
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/112933/1/112933.pdf
http://psasir.upm.edu.my/id/eprint/112933/
https://linkinghub.elsevier.com/retrieve/pii/S0308814601002564
_version_ 1823093028913414144
score 13.239859

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