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Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia

Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting w...

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Main Authors: Waziri, Abdul Aziz, Zulperi, Dzarifah, Ismail, Siti Izera
Format: Article
Published: Penerbit Universiti Kebangsaan Malaysia (UKM Press) 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109219/
http://www.ukm.my/jsm/pdf_files/SM-PDF-52-3-2023/1.pdf
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spelling my.upm.eprints.1092192024-08-27T04:09:41Z http://psasir.upm.edu.my/id/eprint/109219/ Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia Waziri, Abdul Aziz Zulperi, Dzarifah Ismail, Siti Izera Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting watermelon is unknown. This disease decreases the fresh fruit marketability. Watermelon plants cultivar Red Rocky showing varied degree (40-90%) of suspected GSB, were collected from two main watermelon growing areas in Malaysia. Initial symptoms appeared as marginal brown necrotic lesions on leaves, while on the stems showed water-soaked, necrotic lesions and exuded reddish-brown gummy exudate on the stems. A total of ten isolates were isolated from lesions on leaves and stems of watermelon plants affected by GSB. All ten fungal isolates were identified as Stagonosporopsis cucurbitacearum on the basis of morphological characteristics and phylogenetic analysis of combined sequences of the internal transcribed spacer (ITS) and β-tubulin regions. All isolates were proven to be pathogenic when inoculated on the leaves and stems of the watermelon plant and the the fungal isolates were consistently reisolated from the diseased watermelon plants confirming Koch’s postulates. Pathogenicity tests indicated that there were significant differences in virulence among the S. cucurbitacearum when inoculated on the leaves and stems of the watermelon plant. Understanding the etiology of the pathogen will help in disease management of gummy stem blight disease in Malaysia. Penerbit Universiti Kebangsaan Malaysia (UKM Press) 2023 Article PeerReviewed Waziri, Abdul Aziz and Zulperi, Dzarifah and Ismail, Siti Izera (2023) Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia. Sains Malaysiana, 52 (3). pp. 683-691. ISSN 0126-6039; ESSN: 2735-0118 http://www.ukm.my/jsm/pdf_files/SM-PDF-52-3-2023/1.pdf 10.17576/jsm-2023-5203-01
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting watermelon is unknown. This disease decreases the fresh fruit marketability. Watermelon plants cultivar Red Rocky showing varied degree (40-90%) of suspected GSB, were collected from two main watermelon growing areas in Malaysia. Initial symptoms appeared as marginal brown necrotic lesions on leaves, while on the stems showed water-soaked, necrotic lesions and exuded reddish-brown gummy exudate on the stems. A total of ten isolates were isolated from lesions on leaves and stems of watermelon plants affected by GSB. All ten fungal isolates were identified as Stagonosporopsis cucurbitacearum on the basis of morphological characteristics and phylogenetic analysis of combined sequences of the internal transcribed spacer (ITS) and β-tubulin regions. All isolates were proven to be pathogenic when inoculated on the leaves and stems of the watermelon plant and the the fungal isolates were consistently reisolated from the diseased watermelon plants confirming Koch’s postulates. Pathogenicity tests indicated that there were significant differences in virulence among the S. cucurbitacearum when inoculated on the leaves and stems of the watermelon plant. Understanding the etiology of the pathogen will help in disease management of gummy stem blight disease in Malaysia.
format Article
author Waziri, Abdul Aziz
Zulperi, Dzarifah
Ismail, Siti Izera
spellingShingle Waziri, Abdul Aziz
Zulperi, Dzarifah
Ismail, Siti Izera
Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
author_facet Waziri, Abdul Aziz
Zulperi, Dzarifah
Ismail, Siti Izera
author_sort Waziri, Abdul Aziz
title Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_short Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_full Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_fullStr Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_full_unstemmed Molecular characterization and pathogenicity of Stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_sort molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (citrullus lanatus l.) in malaysia
publisher Penerbit Universiti Kebangsaan Malaysia (UKM Press)
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109219/
http://www.ukm.my/jsm/pdf_files/SM-PDF-52-3-2023/1.pdf
_version_ 1809142976853573632
score 13.211869

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