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HIF-1 sensor in detecting hypoxia tolerance at high altitude

INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serv...

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Main Authors: Shaharuddin, Shazreen, Nik Abd Rahman, Nik Mohd Afizan, Masarudin, Mas Jaffri, Alamassi, Mohammedarfat N., Ahmad Saad, Fathinul Fikri
Format: Article
Published: Aerospace Medical Association 2023
Online Access:http://psasir.upm.edu.my/id/eprint/108173/
https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03
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spelling my.upm.eprints.1081732024-09-24T07:18:42Z http://psasir.upm.edu.my/id/eprint/108173/ HIF-1 sensor in detecting hypoxia tolerance at high altitude Shaharuddin, Shazreen Nik Abd Rahman, Nik Mohd Afizan Masarudin, Mas Jaffri Alamassi, Mohammedarfat N. Ahmad Saad, Fathinul Fikri INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia. METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions. RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure. Aerospace Medical Association 2023 Article PeerReviewed Shaharuddin, Shazreen and Nik Abd Rahman, Nik Mohd Afizan and Masarudin, Mas Jaffri and Alamassi, Mohammedarfat N. and Ahmad Saad, Fathinul Fikri (2023) HIF-1 sensor in detecting hypoxia tolerance at high altitude. Aerospace Medicine and Human Performance, 94 (6). pp. 1-6. ISSN 2375-6314; ESSN: 2375-6322 https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03 10.3357/amhp.6166.2023
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia. METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions. RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure.
format Article
author Shaharuddin, Shazreen
Nik Abd Rahman, Nik Mohd Afizan
Masarudin, Mas Jaffri
Alamassi, Mohammedarfat N.
Ahmad Saad, Fathinul Fikri
spellingShingle Shaharuddin, Shazreen
Nik Abd Rahman, Nik Mohd Afizan
Masarudin, Mas Jaffri
Alamassi, Mohammedarfat N.
Ahmad Saad, Fathinul Fikri
HIF-1 sensor in detecting hypoxia tolerance at high altitude
author_facet Shaharuddin, Shazreen
Nik Abd Rahman, Nik Mohd Afizan
Masarudin, Mas Jaffri
Alamassi, Mohammedarfat N.
Ahmad Saad, Fathinul Fikri
author_sort Shaharuddin, Shazreen
title HIF-1 sensor in detecting hypoxia tolerance at high altitude
title_short HIF-1 sensor in detecting hypoxia tolerance at high altitude
title_full HIF-1 sensor in detecting hypoxia tolerance at high altitude
title_fullStr HIF-1 sensor in detecting hypoxia tolerance at high altitude
title_full_unstemmed HIF-1 sensor in detecting hypoxia tolerance at high altitude
title_sort hif-1 sensor in detecting hypoxia tolerance at high altitude
publisher Aerospace Medical Association
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/108173/
https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03
_version_ 1811686003278086144
score 13.211869

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