HIF-1 sensor in detecting hypoxia tolerance at high altitude
INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serv...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Aerospace Medical Association
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108173/ https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03 |
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| Summary: | INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia.
METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions.
RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure. |
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