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Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia

Andrographis paniculata is an erect annual plant belongs to the family of Acanthaceae. This species have been reported to possess medicinal properties such as antiviral, antithrombotic, anticancer, anti-inflammatory and many others. In spite of its significant medicinal importance, fundamental a...

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Main Author: Chia, Sok Hua
Format: Thesis
Language:English
English
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/10703/1/FP_2009_26_A.pdf
http://psasir.upm.edu.my/id/eprint/10703/
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spelling my.upm.eprints.107032013-05-27T07:46:47Z http://psasir.upm.edu.my/id/eprint/10703/ Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia Chia, Sok Hua Andrographis paniculata is an erect annual plant belongs to the family of Acanthaceae. This species have been reported to possess medicinal properties such as antiviral, antithrombotic, anticancer, anti-inflammatory and many others. In spite of its significant medicinal importance, fundamental aspects regarding reproductive biology, mating system and genetic diversity of A. paniculata has been poorly investigated. The objectives of this study are to (a) determine the reproductive biology which includes the occurrence of stigma receptivity, anther dehiscence and pollen viability (b) determine the occurrence of naturally self and/or cross pollination and (c) elucidate the genetic diversity of A. paniculata germplasm in Peninsular Malaysia using molecular marker. Reproductive biology of A. paniculata was studied by investigating floral development, stigmatic receptivity, anther dehiscence and pollen viability. Two pollination treatments namely bagged and unbagged were imposed on seven accessions to study the mating system through self-compatibility and inbreeding depression. A total of 80 accessions of A. paniculata representing six different states, namely Perak, Selangor, Negeri Sembilan, Kelantan, Terengganu and Pahang were subjected to Random Amplified Polymorphic DNA (RAPD) assay using 10 primers to elucidate genetic diversity of this plant in Peninsular Malaysia. Results showed that floral development of A. paniculata can be categorised into five stages based upon flower buds length associated with timing of anther dehiscence and stigma receptivity. The anthers started to dehisce when flower buds reached the size of 11.5 mm (Stage III) and stigma became fully receptive at 12 mm (Stage IV). Both events occurred almost simultaneously when corolla still remained close. In addition, stigma of A. paniculata was closely attached to anthers from stage II until flower opening. Thereby, the mode of mating system of A. paniculata can be defined as pollination in flower bud before anthesis, also known as preanthesis cleistogamy. This mechanism of mating system greatly promotes the occurrence of autonomous self-pollination. 2009-10 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/10703/1/FP_2009_26_A.pdf Chia, Sok Hua (2009) Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Andrographis paniculata is an erect annual plant belongs to the family of Acanthaceae. This species have been reported to possess medicinal properties such as antiviral, antithrombotic, anticancer, anti-inflammatory and many others. In spite of its significant medicinal importance, fundamental aspects regarding reproductive biology, mating system and genetic diversity of A. paniculata has been poorly investigated. The objectives of this study are to (a) determine the reproductive biology which includes the occurrence of stigma receptivity, anther dehiscence and pollen viability (b) determine the occurrence of naturally self and/or cross pollination and (c) elucidate the genetic diversity of A. paniculata germplasm in Peninsular Malaysia using molecular marker. Reproductive biology of A. paniculata was studied by investigating floral development, stigmatic receptivity, anther dehiscence and pollen viability. Two pollination treatments namely bagged and unbagged were imposed on seven accessions to study the mating system through self-compatibility and inbreeding depression. A total of 80 accessions of A. paniculata representing six different states, namely Perak, Selangor, Negeri Sembilan, Kelantan, Terengganu and Pahang were subjected to Random Amplified Polymorphic DNA (RAPD) assay using 10 primers to elucidate genetic diversity of this plant in Peninsular Malaysia. Results showed that floral development of A. paniculata can be categorised into five stages based upon flower buds length associated with timing of anther dehiscence and stigma receptivity. The anthers started to dehisce when flower buds reached the size of 11.5 mm (Stage III) and stigma became fully receptive at 12 mm (Stage IV). Both events occurred almost simultaneously when corolla still remained close. In addition, stigma of A. paniculata was closely attached to anthers from stage II until flower opening. Thereby, the mode of mating system of A. paniculata can be defined as pollination in flower bud before anthesis, also known as preanthesis cleistogamy. This mechanism of mating system greatly promotes the occurrence of autonomous self-pollination.
format Thesis
author Chia, Sok Hua
spellingShingle Chia, Sok Hua
Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
author_facet Chia, Sok Hua
author_sort Chia, Sok Hua
title Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
title_short Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
title_full Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
title_fullStr Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
title_full_unstemmed Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
title_sort reproductive system and genetic diversity of hempedu bumi (andrographis paniculata) germplasm in peninsular malaysia
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/10703/1/FP_2009_26_A.pdf
http://psasir.upm.edu.my/id/eprint/10703/
_version_ 1643824682505338880
score 13.211869

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