Reproductive System and Genetic Diversity of Hempedu Bumi (Andrographis Paniculata) Germplasm in Peninsular Malaysia
Andrographis paniculata is an erect annual plant belongs to the family of Acanthaceae. This species have been reported to possess medicinal properties such as antiviral, antithrombotic, anticancer, anti-inflammatory and many others. In spite of its significant medicinal importance, fundamental a...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/10703/1/FP_2009_26_A.pdf http://psasir.upm.edu.my/id/eprint/10703/ |
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| Summary: | Andrographis paniculata is an erect annual plant belongs to the family of
Acanthaceae. This species have been reported to possess medicinal
properties such as antiviral, antithrombotic, anticancer, anti-inflammatory and
many others. In spite of its significant medicinal importance, fundamental
aspects regarding reproductive biology, mating system and genetic diversity
of A. paniculata has been poorly investigated. The objectives of this study are
to (a) determine the reproductive biology which includes the occurrence of
stigma receptivity, anther dehiscence and pollen viability (b) determine the
occurrence of naturally self and/or cross pollination and (c) elucidate the
genetic diversity of A. paniculata germplasm in Peninsular Malaysia using
molecular marker. Reproductive biology of A. paniculata was studied by investigating floral
development, stigmatic receptivity, anther dehiscence and pollen viability.
Two pollination treatments namely bagged and unbagged were imposed on
seven accessions to study the mating system through self-compatibility and
inbreeding depression. A total of 80 accessions of A. paniculata representing
six different states, namely Perak, Selangor, Negeri Sembilan, Kelantan,
Terengganu and Pahang were subjected to Random Amplified Polymorphic
DNA (RAPD) assay using 10 primers to elucidate genetic diversity of this
plant in Peninsular Malaysia.
Results showed that floral development of A. paniculata can be categorised
into five stages based upon flower buds length associated with timing of
anther dehiscence and stigma receptivity. The anthers started to dehisce
when flower buds reached the size of 11.5 mm (Stage III) and stigma
became fully receptive at 12 mm (Stage IV). Both events occurred almost
simultaneously when corolla still remained close. In addition, stigma of A.
paniculata was closely attached to anthers from stage II until flower opening.
Thereby, the mode of mating system of A. paniculata can be defined as
pollination in flower bud before anthesis, also known as preanthesis
cleistogamy. This mechanism of mating system greatly promotes the
occurrence of autonomous self-pollination. |
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