Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002

Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding...

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Main Authors: Muhammad Suhaib, M. H., Mohd Hasnain, Hussain, Awang Ahmad Sallehin, Awang Husaini, Kopli, Bujang, Dayang Salwani, Awang Adeni, Mohd Azib, Salleh
Format: Article
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2013
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Online Access:http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf
http://ir.unimas.my/id/eprint/9829/
http://www.researchgate.net/publication/280813911
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spelling my.unimas.ir.98292022-01-25T07:33:28Z http://ir.unimas.my/id/eprint/9829/ Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 Muhammad Suhaib, M. H. Mohd Hasnain, Hussain Awang Ahmad Sallehin, Awang Husaini Kopli, Bujang Dayang Salwani, Awang Adeni Mohd Azib, Salleh Q Science (General) QR Microbiology Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23 Universiti Malaysia Sarawak, (UNIMAS) 2013 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf Muhammad Suhaib, M. H. and Mohd Hasnain, Hussain and Awang Ahmad Sallehin, Awang Husaini and Kopli, Bujang and Dayang Salwani, Awang Adeni and Mohd Azib, Salleh (2013) Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002. Borneo Journal of Resource Science and Technology, 3 (2). pp. 36-39. ISSN 229-9769 http://www.researchgate.net/publication/280813911
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Muhammad Suhaib, M. H.
Mohd Hasnain, Hussain
Awang Ahmad Sallehin, Awang Husaini
Kopli, Bujang
Dayang Salwani, Awang Adeni
Mohd Azib, Salleh
Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
description Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23
format Article
author Muhammad Suhaib, M. H.
Mohd Hasnain, Hussain
Awang Ahmad Sallehin, Awang Husaini
Kopli, Bujang
Dayang Salwani, Awang Adeni
Mohd Azib, Salleh
author_facet Muhammad Suhaib, M. H.
Mohd Hasnain, Hussain
Awang Ahmad Sallehin, Awang Husaini
Kopli, Bujang
Dayang Salwani, Awang Adeni
Mohd Azib, Salleh
author_sort Muhammad Suhaib, M. H.
title Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
title_short Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
title_full Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
title_fullStr Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
title_full_unstemmed Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
title_sort preliminary gene characterization of α-amylase from bacillus amyloliquefaciens umas 1002
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2013
url http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf
http://ir.unimas.my/id/eprint/9829/
http://www.researchgate.net/publication/280813911
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