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Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products

A sensitive and selective reversed phase HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl me...

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Main Authors: Fasihuddin Badruddin, Ahmad, Mohammed, Akkbik, Zaini Bin, Assim
Format: E-Article
Published: 2012
Subjects:
Q Science (General)
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/7961/
http://eurasianjournals.com/index.php/ejac/article/viewFile/594/pdf_59
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spelling my.unimas.ir.79612015-06-24T04:01:06Z http://ir.unimas.my/id/eprint/7961/ Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products Fasihuddin Badruddin, Ahmad Mohammed, Akkbik Zaini Bin, Assim Q Science (General) R Medicine (General) A sensitive and selective reversed phase HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamat (OMC) as UVB-filter in several personal care products. The optimum analytical conditions (280 nm as maximum wave length, 0.8 mL/min as flow rate, pH 3.5, mixture of phase A (acetonitrile) with phase B (water:acetic acid; 99:1; v/v) as mobile phase) were obtained by variation of flow rate, the pH value of phase B, different binary mixtures. The dynamic range was between 1 to 250 mg L-1 with relative standard deviation better than 0.25%, (n=4). Limit of detection for BHA, BHT and OMC were 0.196, 0.170 and 0.478 mg L-1, respectively. While limit of quantification for BHA, BHT and OMC were 0.593, 0.515 and 1.448 mg L-1, respectively. Recovery study was performed by spiking standard of phenolic compounds at four different concentration levels using external standard addition method. The recovery for BHA, BHT and OMC were ranged from 92.1-105.9%, 83.2-108.9% and 87.3-103.7%, respectively. The concentration ranges of BHA, BHT and OMC in 12 commercial personal care samples were 0.13-4.85, 0.16-2.30 and 0.12-65.5 mg g-1, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation i.e up to 1% (w/w) for BHA, up to 0.5% (w/w) for BHT and up to 10% (w/w) for OMC 2012 E-Article PeerReviewed Fasihuddin Badruddin, Ahmad and Mohammed, Akkbik and Zaini Bin, Assim (2012) Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products. Eurasian Journal of Analytical Chemistry, 8 (2). pp. 79-89. ISSN 1306-3057 http://eurasianjournals.com/index.php/ejac/article/viewFile/594/pdf_59 DOI: 10.1016/j.actatropica.2013.11.001
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
topic Q Science (General)
R Medicine (General)
spellingShingle Q Science (General)
R Medicine (General)
Fasihuddin Badruddin, Ahmad
Mohammed, Akkbik
Zaini Bin, Assim
Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
description A sensitive and selective reversed phase HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamat (OMC) as UVB-filter in several personal care products. The optimum analytical conditions (280 nm as maximum wave length, 0.8 mL/min as flow rate, pH 3.5, mixture of phase A (acetonitrile) with phase B (water:acetic acid; 99:1; v/v) as mobile phase) were obtained by variation of flow rate, the pH value of phase B, different binary mixtures. The dynamic range was between 1 to 250 mg L-1 with relative standard deviation better than 0.25%, (n=4). Limit of detection for BHA, BHT and OMC were 0.196, 0.170 and 0.478 mg L-1, respectively. While limit of quantification for BHA, BHT and OMC were 0.593, 0.515 and 1.448 mg L-1, respectively. Recovery study was performed by spiking standard of phenolic compounds at four different concentration levels using external standard addition method. The recovery for BHA, BHT and OMC were ranged from 92.1-105.9%, 83.2-108.9% and 87.3-103.7%, respectively. The concentration ranges of BHA, BHT and OMC in 12 commercial personal care samples were 0.13-4.85, 0.16-2.30 and 0.12-65.5 mg g-1, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation i.e up to 1% (w/w) for BHA, up to 0.5% (w/w) for BHT and up to 10% (w/w) for OMC
format E-Article
author Fasihuddin Badruddin, Ahmad
Mohammed, Akkbik
Zaini Bin, Assim
author_facet Fasihuddin Badruddin, Ahmad
Mohammed, Akkbik
Zaini Bin, Assim
author_sort Fasihuddin Badruddin, Ahmad
title Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
title_short Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
title_full Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
title_fullStr Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
title_full_unstemmed Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
title_sort optimization and validation of rp-hplc-uv/vis method for determination phenolic compounds in several personal care products
publishDate 2012
url http://ir.unimas.my/id/eprint/7961/
http://eurasianjournals.com/index.php/ejac/article/viewFile/594/pdf_59
_version_ 1644510421683535872
score 13.211869

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