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Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste

Amylase is one of the most important enzymes in industrial application. The study investigated the production and characterization of produced by NSH9 isolates under solid state fermentation. The study showed cassava was the best substrate for amylase inducer. The highest amylase production detecte...

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Main Author: Awang Ahmad Tarmizi, Awang Habeni
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/7882/1/Awang%20Ahmad%20Tarmizi%20bin%20Awang%20Habeni%20ft.pdf
http://ir.unimas.my/id/eprint/7882/
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spelling my.unimas.ir.78822023-02-27T04:03:29Z http://ir.unimas.my/id/eprint/7882/ Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste Awang Ahmad Tarmizi, Awang Habeni SB Plant culture Amylase is one of the most important enzymes in industrial application. The study investigated the production and characterization of produced by NSH9 isolates under solid state fermentation. The study showed cassava was the best substrate for amylase inducer. The highest amylase production detected at 72 hours of incubation with enzyme activity is 4.3170 IU/ml and the protein concentration was 0.0104 mg/ml. The best pH for amylase production was pH7.0 with 4.3170 IU/ml and 4.3170 IU/ml for 27°C of incubation temperature. The protein concentration for pH and temperature was 0.0104 mg/ml and 0.0104 mg/ml. The enzyme was analyzed by using dinitrosalicyclic acid (DNS) assay method. The characteristic of enzyme activity was showed the highest activity at pH6 (1.7325 IU/ml) and at 37°C (1.6998 IU/ml) after 10 minutes incubated with 1% (w/v) soluble starch. The kinetic study of the amylase produced was shown the value of Vmax is 0.4743 mg ml-1min-1 and Km is 679.86 mg ml-1. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7882/1/Awang%20Ahmad%20Tarmizi%20bin%20Awang%20Habeni%20ft.pdf Awang Ahmad Tarmizi, Awang Habeni (2010) Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Awang Ahmad Tarmizi, Awang Habeni
Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
description Amylase is one of the most important enzymes in industrial application. The study investigated the production and characterization of produced by NSH9 isolates under solid state fermentation. The study showed cassava was the best substrate for amylase inducer. The highest amylase production detected at 72 hours of incubation with enzyme activity is 4.3170 IU/ml and the protein concentration was 0.0104 mg/ml. The best pH for amylase production was pH7.0 with 4.3170 IU/ml and 4.3170 IU/ml for 27°C of incubation temperature. The protein concentration for pH and temperature was 0.0104 mg/ml and 0.0104 mg/ml. The enzyme was analyzed by using dinitrosalicyclic acid (DNS) assay method. The characteristic of enzyme activity was showed the highest activity at pH6 (1.7325 IU/ml) and at 37°C (1.6998 IU/ml) after 10 minutes incubated with 1% (w/v) soluble starch. The kinetic study of the amylase produced was shown the value of Vmax is 0.4743 mg ml-1min-1 and Km is 679.86 mg ml-1.
format Final Year Project Report
author Awang Ahmad Tarmizi, Awang Habeni
author_facet Awang Ahmad Tarmizi, Awang Habeni
author_sort Awang Ahmad Tarmizi, Awang Habeni
title Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
title_short Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
title_full Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
title_fullStr Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
title_full_unstemmed Optimization and Characterization of x-Amylase Produced by Raw Starch Degrading Fungi Isolate NSH9 Using Solid State Fermentation (SSF) of Agriculture Waste
title_sort optimization and characterization of x-amylase produced by raw starch degrading fungi isolate nsh9 using solid state fermentation (ssf) of agriculture waste
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2010
url http://ir.unimas.my/id/eprint/7882/1/Awang%20Ahmad%20Tarmizi%20bin%20Awang%20Habeni%20ft.pdf
http://ir.unimas.my/id/eprint/7882/
_version_ 1758970229445099520
score 13.211869

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