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Development of test method for anti-amoebic activity

This study was carried out to obtain preliminary data of anti-amoebic screening test using non-pathogenic free living amoebae (FLA) and thus develop an appropriate preliminary test method for anti-amoebic activity. The anti-amoebic screening test was performed by treating free living amoebae with...

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Bibliographic Details
Main Author: Boo, Chie Yi
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
GE Environmental Sciences
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/7365/1/Boo%20Chie%20Yi%20ft.pdf
http://ir.unimas.my/id/eprint/7365/
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spelling my.unimas.ir.73652023-02-14T07:08:30Z http://ir.unimas.my/id/eprint/7365/ Development of test method for anti-amoebic activity Boo, Chie Yi GE Environmental Sciences Q Science (General) This study was carried out to obtain preliminary data of anti-amoebic screening test using non-pathogenic free living amoebae (FLA) and thus develop an appropriate preliminary test method for anti-amoebic activity. The anti-amoebic screening test was performed by treating free living amoebae with amino acid-thiourea derivative at concentration of 50 µg/mL. The viability of amoebae was examined and compared with the treatment thiourea control and NaCl cytotoxicity control at the same concentration. The number of viable FLA was calculated from the first 50 FLA that observed using inverted microscope with 100x magnification. One-way ANOVA was used to statistically analyse the data and Fisher‟s Least Significant Difference (LSD) test was conducted to analyse the significant data. Results of amino acid-thiourea derivative treatment showed potential anti-amoebic activity against non-pathogenic FLA. Test model of anti-amoebic activity was established and further studies can be carried out by using the same compound at different concentration levels. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7365/1/Boo%20Chie%20Yi%20ft.pdf Boo, Chie Yi (2012) Development of test method for anti-amoebic activity. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
Q Science (General)
spellingShingle GE Environmental Sciences
Q Science (General)
Boo, Chie Yi
Development of test method for anti-amoebic activity
description This study was carried out to obtain preliminary data of anti-amoebic screening test using non-pathogenic free living amoebae (FLA) and thus develop an appropriate preliminary test method for anti-amoebic activity. The anti-amoebic screening test was performed by treating free living amoebae with amino acid-thiourea derivative at concentration of 50 µg/mL. The viability of amoebae was examined and compared with the treatment thiourea control and NaCl cytotoxicity control at the same concentration. The number of viable FLA was calculated from the first 50 FLA that observed using inverted microscope with 100x magnification. One-way ANOVA was used to statistically analyse the data and Fisher‟s Least Significant Difference (LSD) test was conducted to analyse the significant data. Results of amino acid-thiourea derivative treatment showed potential anti-amoebic activity against non-pathogenic FLA. Test model of anti-amoebic activity was established and further studies can be carried out by using the same compound at different concentration levels.
format Final Year Project Report
author Boo, Chie Yi
author_facet Boo, Chie Yi
author_sort Boo, Chie Yi
title Development of test method for anti-amoebic activity
title_short Development of test method for anti-amoebic activity
title_full Development of test method for anti-amoebic activity
title_fullStr Development of test method for anti-amoebic activity
title_full_unstemmed Development of test method for anti-amoebic activity
title_sort development of test method for anti-amoebic activity
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2012
url http://ir.unimas.my/id/eprint/7365/1/Boo%20Chie%20Yi%20ft.pdf
http://ir.unimas.my/id/eprint/7365/
_version_ 1758582477659570176
score 13.211869

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