Development of test method for anti-amoebic activity
This study was carried out to obtain preliminary data of anti-amoebic screening test using non-pathogenic free living amoebae (FLA) and thus develop an appropriate preliminary test method for anti-amoebic activity. The anti-amoebic screening test was performed by treating free living amoebae with...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7365/1/Boo%20Chie%20Yi%20ft.pdf http://ir.unimas.my/id/eprint/7365/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study was carried out to obtain preliminary data of anti-amoebic screening test using
non-pathogenic free living amoebae (FLA) and thus develop an appropriate preliminary test
method for anti-amoebic activity. The anti-amoebic screening test was performed by treating
free living amoebae with amino acid-thiourea derivative at concentration of 50 µg/mL. The
viability of amoebae was examined and compared with the treatment thiourea control and
NaCl cytotoxicity control at the same concentration. The number of viable FLA was
calculated from the first 50 FLA that observed using inverted microscope with 100x
magnification. One-way ANOVA was used to statistically analyse the data and Fisher‟s Least
Significant Difference (LSD) test was conducted to analyse the significant data. Results of
amino acid-thiourea derivative treatment showed potential anti-amoebic activity against
non-pathogenic FLA. Test model of anti-amoebic activity was established and further
studies can be carried out by using the same compound at different concentration levels. |
|---|