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Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity

A newly isolated lactic acid bacteria (LAB) from laboratory of Biofuel R&D (UNIMAS) that has the capability to produce lactic acid (LA) using hydrolysed sago starch as the only carbon source by fermentation process was identified biochemically and it’s fermentative capacity was studied by usin...

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Main Author: Revathy, D/O Sankaran.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2013
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/4447/1/Revathy%20full.pdf
http://ir.unimas.my/id/eprint/4447/
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spelling my.unimas.ir.44472024-03-14T06:17:37Z http://ir.unimas.my/id/eprint/4447/ Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity Revathy, D/O Sankaran. Q Science (General) QR Microbiology A newly isolated lactic acid bacteria (LAB) from laboratory of Biofuel R&D (UNIMAS) that has the capability to produce lactic acid (LA) using hydrolysed sago starch as the only carbon source by fermentation process was identified biochemically and it’s fermentative capacity was studied by using batch fermentation. The strain was tested to ferment for some carbohydrates contained biochemical kit test API 20E. The isolated LAB was used for LA fermentation production. The production biomass and LA production of the LAB strain were studied with two conditions which were at pH7 and pH8 at temperature of 37°C. From this study, the strain was identified as Enterococcus faecalis that is able to produce high concentration of LA at pH 8 which is very useful for industrial application. Fermentation at pH 8 produced a maximum dry cell weight of about 8.48g/L and 130.03g/L of LA, while less production was shown at pH 7 with 5.83g/L and 69g/L. It has been found that the growth of biomass and LA production for Enterococcus faecalis are influenced by the pH and the optimum pH for this strain is found to be at pH8. Universiti Malaysia Sarawak (UNIMAS) 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/4447/1/Revathy%20full.pdf Revathy, D/O Sankaran. (2013) Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Revathy, D/O Sankaran.
Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
description A newly isolated lactic acid bacteria (LAB) from laboratory of Biofuel R&D (UNIMAS) that has the capability to produce lactic acid (LA) using hydrolysed sago starch as the only carbon source by fermentation process was identified biochemically and it’s fermentative capacity was studied by using batch fermentation. The strain was tested to ferment for some carbohydrates contained biochemical kit test API 20E. The isolated LAB was used for LA fermentation production. The production biomass and LA production of the LAB strain were studied with two conditions which were at pH7 and pH8 at temperature of 37°C. From this study, the strain was identified as Enterococcus faecalis that is able to produce high concentration of LA at pH 8 which is very useful for industrial application. Fermentation at pH 8 produced a maximum dry cell weight of about 8.48g/L and 130.03g/L of LA, while less production was shown at pH 7 with 5.83g/L and 69g/L. It has been found that the growth of biomass and LA production for Enterococcus faecalis are influenced by the pH and the optimum pH for this strain is found to be at pH8.
format Final Year Project Report
author Revathy, D/O Sankaran.
author_facet Revathy, D/O Sankaran.
author_sort Revathy, D/O Sankaran.
title Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
title_short Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
title_full Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
title_fullStr Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
title_full_unstemmed Biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
title_sort biochemical identification of an alkalophilic lactic acid bacterium and testing its fermentative capacity
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2013
url http://ir.unimas.my/id/eprint/4447/1/Revathy%20full.pdf
http://ir.unimas.my/id/eprint/4447/
_version_ 1794643950638727168
score 13.211869

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