Fish Analysis Containing Biogenic Amines Using Gas Chromatography Equipped With Flame Ionization And Mass Spectrometer Detectors

Biogenic amines generally can be found in fish due to amines in fish undergoing a degradation process. According to the United States Food and Drug Administration (FDA), biogenic amines in fish and fish products can cause harm to consumers if consumed more than 50 µg/mL. Thus, it is important to ana...

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Bibliographic Details
Main Authors: Ahlam, Inayatullah, Hamid Alkhair, Badrul, Muhammad Abdurrahman, Munir
Format: Article
Language:English
Published: Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya 2021
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Online Access:http://ir.unimas.my/id/eprint/43926/3/Fish%20Analysis.pdf
http://ir.unimas.my/id/eprint/43926/
https://sciencetechindonesia.com/index.php/jsti/article/view/260#:~:text=Five%20biogenic%20amines%20such%20as,biogenic%20amines%20structures%20were%20confirmed
https://doi.org/10.26554/sti.2021.6.1.1-7
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Summary:Biogenic amines generally can be found in fish due to amines in fish undergoing a degradation process. According to the United States Food and Drug Administration (FDA), biogenic amines in fish and fish products can cause harm to consumers if consumed more than 50 µg/mL. Thus, it is important to analyze them. Five biogenic amines such as heptylamine, histamine, tyramine, cadaverine and spermidine were extracted using soaking method with methanol 50% (v/v), a�erward they were detected in fish and fish products using gas chromatography – flame ionisation detector (GC-FID) and the biogenic amines structures were confirmed using mass spectrometry (MS). The detection limits (DLs) were range at 1.20 – 2.90 µg/mL. Histamine was detected in fish and fish products such as sardine (Sardinella gibosa) and mackerel (Scomberomorus guttatus) at concentration of 5.96 and 2.69 µg/mL, respectively, whereas cadaverine was found in sardine (Sardinella gibosa) at concentration of 4.96 µg/mL. Histamine concentrations in this study were detected below 50 µg/mL which is below the permissible threshold associated with scombroid poisoning.