Enzymatic hydrolysis of spent Saccharomyces cerevisiae derived from sago bioethanol fermentation
Spent Saccharomyces cerevisiae is a by-product of bioethanol fermentation. The spent yeast is abundant in valuable components which can be used for many applications. One of the ways to prepare yeast extract is through enzymatic hydrolysis which is by rupturing the yeast cell walls using e...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Researchers Associations,
2023
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/43696/1/paper%202-published-1.pdf http://ir.unimas.my/id/eprint/43696/ https://worldresearchersassociations.com/Archives/RJBT/Vol(18)2023/June%202023/Enzymatic%20hydrolysis%20of%20spent%20Saccharomyces%20cerevisiae%20derived%20from%20sago%20bioethanol%20fermentation.aspx https://doi.org/10.25303/1806rjbt01009 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Spent Saccharomyces cerevisiae is a by-product of
bioethanol fermentation. The spent yeast is abundant in
valuable components which can be used for many
applications. One of the ways to prepare yeast extract
is through enzymatic hydrolysis which is by rupturing
the yeast cell walls using exogenous enzymes under
certain conditions that promote the leakage of
intracellular compounds. To date, enzymatic
hydrolysis of spent S. cerevisiae derived from the
production of sago bioethanol is yet to be thoroughly
explored. In the present study, we examine the
feasibility of enzymatic hydrolysis of spent S. cerevisiae
generated from sago bioethanol fermentation. The
effect of two enzymes namely alcalase and cellulase
and their concentrations (0.1-0.5% (v/v) on the release
of protein and carbohydrate in the hydrolysate was
also investigated.
Additionally, the surface morphology of the hydrolysed
yeast cells was observed using a Scanning electron
microscope (SEM). Our results showed that the optimal
concentration of alcalase and cellulase for enzymatic
hydrolysis of spent S. cerevisiae was 0.4% (v/v) and
0.5% (v/v) respectively. In addition, cellulase was
found to be more superior than alcalase with respect to
the protein content in the hydrolysate. The enzymatic
hydrolysis of spent yeast by alcalase and cellulase
yielded improvements of 1.1 to 1.8-fold and 3.5 to 5.6-
fold of protein and total carbohydrate concentration
respectively in comparison to that achieved via
autolysis. It was evident from the SEM analysis that
there was a notable change in the surface morphology
of the lysed yeast cells indicating the lysis of the yeast
cells throughout the enzymatic hydrolysis. In summary,
the current work provides useful insights into the
strategies of valorising spent S. cerevisiae generated
from sago bioethanol production. This will further help
the development of value-added products from the
waste, hence promoting a sustainable economy besides
reducing the environmental impacts associated with
the disposal of spent S. cerevisiae. |
|---|