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Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau

Amylases are starch-degrading enzymes that catalyzes the breakdown of glycosidic linkages in low molecular weight molecules like glucose and maltose. To date many studies regarding bacteria have been isolated from the environment that can be used in many industries. The purpose of current research w...

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Main Author: Nur Ain Fatihah, Ahmad Talahah
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/39637/1/NUR%20AIN%20FATIHAH%20BINTI%20AHMAD%20TALAHAH%20ft.pdf
http://ir.unimas.my/id/eprint/39637/
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spelling my.unimas.ir.396372022-09-12T07:17:38Z http://ir.unimas.my/id/eprint/39637/ Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau Nur Ain Fatihah, Ahmad Talahah QR Microbiology Amylases are starch-degrading enzymes that catalyzes the breakdown of glycosidic linkages in low molecular weight molecules like glucose and maltose. To date many studies regarding bacteria have been isolated from the environment that can be used in many industries. The purpose of current research was to screen amylase enzymes activity of bacteria producing amylase from soil samples near Wind cave, Bau and. The submerged fermentation approach is applied to produce amylase at room temperature for 48 hours. It was determined that the amylase activity could be estimated using the supernatant that was obtained. The DNSA approach was utilized to perform an amylase activity assay by determining the amount of reducing sugar. A total of 9 isolates were obtained after performing the spread plate technique. Two of the isolates, FB2 and FB3 displayed a positive response for amylase enzyme by the development of a clear zone of starch hydrolysis on starch agar. It was perceived that isolate FB2 and FB3 exhibiting amylase activity of 6.3024 and 4.2145 U/ml respectively. However, additional exploration is required in order to evaluate the characteristics of the enzyme, as well as its pH stability and the influence of various parameters. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39637/1/NUR%20AIN%20FATIHAH%20BINTI%20AHMAD%20TALAHAH%20ft.pdf Nur Ain Fatihah, Ahmad Talahah (2022) Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Nur Ain Fatihah, Ahmad Talahah
Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
description Amylases are starch-degrading enzymes that catalyzes the breakdown of glycosidic linkages in low molecular weight molecules like glucose and maltose. To date many studies regarding bacteria have been isolated from the environment that can be used in many industries. The purpose of current research was to screen amylase enzymes activity of bacteria producing amylase from soil samples near Wind cave, Bau and. The submerged fermentation approach is applied to produce amylase at room temperature for 48 hours. It was determined that the amylase activity could be estimated using the supernatant that was obtained. The DNSA approach was utilized to perform an amylase activity assay by determining the amount of reducing sugar. A total of 9 isolates were obtained after performing the spread plate technique. Two of the isolates, FB2 and FB3 displayed a positive response for amylase enzyme by the development of a clear zone of starch hydrolysis on starch agar. It was perceived that isolate FB2 and FB3 exhibiting amylase activity of 6.3024 and 4.2145 U/ml respectively. However, additional exploration is required in order to evaluate the characteristics of the enzyme, as well as its pH stability and the influence of various parameters.
format Final Year Project Report
author Nur Ain Fatihah, Ahmad Talahah
author_facet Nur Ain Fatihah, Ahmad Talahah
author_sort Nur Ain Fatihah, Ahmad Talahah
title Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
title_short Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
title_full Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
title_fullStr Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
title_full_unstemmed Screening of Amylase Activity of Bacteria Isolated from Soil Sample from Wind Cave, Bau
title_sort screening of amylase activity of bacteria isolated from soil sample from wind cave, bau
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39637/1/NUR%20AIN%20FATIHAH%20BINTI%20AHMAD%20TALAHAH%20ft.pdf
http://ir.unimas.my/id/eprint/39637/
_version_ 1744357787876458496
score 13.211869

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