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Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian

Durian (Durio zibethinus), is a flowering plant in the Bombacaceae family. Currently, the conventional breeding method is unable to fulfill the increasing demand of Musang King durians due to being relatively time-consuming. Thus, propagation through the tissue culture technique can increase the p...

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Bibliographic Details
Main Author: Tang, Ye-Fang
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
Q Science (General)
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/35376/1/Optimization%20of%20Surface%20Sterilization%20Protocol%20by%20Using%20Bleach%20for%20Musang%20King%20Durian%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35376/5/Tang%20Ye-Fang.pdf
http://ir.unimas.my/id/eprint/35376/
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spelling my.unimas.ir.353762024-03-21T01:45:41Z http://ir.unimas.my/id/eprint/35376/ Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian Tang, Ye-Fang Q Science (General) SB Plant culture Durian (Durio zibethinus), is a flowering plant in the Bombacaceae family. Currently, the conventional breeding method is unable to fulfill the increasing demand of Musang King durians due to being relatively time-consuming. Thus, propagation through the tissue culture technique can increase the production and meet increased market demand. The application of tissue culture requires sterile and living explants. The objective of this study was to investigate the optimal surface sterilization techniques for the leaves ofMusang King. The leaves from the grafted Musang King seedlings were sterilized by using different concentrations of Clorox (0 % (control), 30 %, 40 %, 50 % and 60 %) with different exposure times (40 and 50 minutes). The percentage of sterile explants and the percentage of browning explants were recorded at 7, 14 and 21 days after culture. All treatments were carried out in three replicates using five samples for each replication. The result showed that using 60 % Clorox for 50 minutes, which gave the highest percentage of sterile explants, 40.00±52.92 %, while the treatment with 40 % of Clorox for 40 minutes gave the lowest percentage of sterile explants, which was 6.67±11.55 %. The 60 % of Clorox for 40 minutes had 6.67±11.55 % of browning explants. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35376/1/Optimization%20of%20Surface%20Sterilization%20Protocol%20by%20Using%20Bleach%20for%20Musang%20King%20Durian%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/35376/5/Tang%20Ye-Fang.pdf Tang, Ye-Fang (2018) Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
SB Plant culture
spellingShingle Q Science (General)
SB Plant culture
Tang, Ye-Fang
Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
description Durian (Durio zibethinus), is a flowering plant in the Bombacaceae family. Currently, the conventional breeding method is unable to fulfill the increasing demand of Musang King durians due to being relatively time-consuming. Thus, propagation through the tissue culture technique can increase the production and meet increased market demand. The application of tissue culture requires sterile and living explants. The objective of this study was to investigate the optimal surface sterilization techniques for the leaves ofMusang King. The leaves from the grafted Musang King seedlings were sterilized by using different concentrations of Clorox (0 % (control), 30 %, 40 %, 50 % and 60 %) with different exposure times (40 and 50 minutes). The percentage of sterile explants and the percentage of browning explants were recorded at 7, 14 and 21 days after culture. All treatments were carried out in three replicates using five samples for each replication. The result showed that using 60 % Clorox for 50 minutes, which gave the highest percentage of sterile explants, 40.00±52.92 %, while the treatment with 40 % of Clorox for 40 minutes gave the lowest percentage of sterile explants, which was 6.67±11.55 %. The 60 % of Clorox for 40 minutes had 6.67±11.55 % of browning explants.
format Final Year Project Report
author Tang, Ye-Fang
author_facet Tang, Ye-Fang
author_sort Tang, Ye-Fang
title Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
title_short Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
title_full Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
title_fullStr Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
title_full_unstemmed Optimization of Surface Sterilization Protocol by Using Bleach for Musang King Durian
title_sort optimization of surface sterilization protocol by using bleach for musang king durian
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35376/1/Optimization%20of%20Surface%20Sterilization%20Protocol%20by%20Using%20Bleach%20for%20Musang%20King%20Durian%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35376/5/Tang%20Ye-Fang.pdf
http://ir.unimas.my/id/eprint/35376/
_version_ 1794644142423277568
score 13.211869

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