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Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm

Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri, Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However, their...

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Bibliographic Details
Main Author: Stephanie Lenja, Sait
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS) 2018
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/35179/1/Stephanie%20Lenja%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35179/4/Stephanie%20Lenja%20ft.pdf
http://ir.unimas.my/id/eprint/35179/
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spelling my.unimas.ir.351792024-08-14T03:58:13Z http://ir.unimas.my/id/eprint/35179/ Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm Stephanie Lenja, Sait Q Science (General) Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri, Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However, their presences in aquaculture sector had become a concern, especially affect towards to human health. Besides, treatment of shigellosis with appropriate antimicrobial agents is able to shorten duration of illness the consequences are they able to resists against antimicrobial agents are increasing. The general aim for this study was to isolate and identification the presences of Shigella spp. in both traditional and commercial aquaculture farm. In order to achieve this, water sampling collection from I traditional pond and 2 aquaculture pond, isolated by XLD agar, differentiated by several biochemical tests and molecular analysis (polymerase chain reaction). Amplification of Shiga toxin (stx gene) was done to confirm the presences of pathogenic Shigella spp. in both type farms. Three cultures samples from each aquaculture farm were assessed with some appropriate antibiotics such as ampicillin, tetracycline, sulphonamides, erythromycin and sulfamethoxazole. Overall, the bacterial strain are able to resist the antibiotics. Universiti Malaysia Sarawak (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35179/1/Stephanie%20Lenja%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/35179/4/Stephanie%20Lenja%20ft.pdf Stephanie Lenja, Sait (2018) Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Stephanie Lenja, Sait
Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
description Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri, Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However, their presences in aquaculture sector had become a concern, especially affect towards to human health. Besides, treatment of shigellosis with appropriate antimicrobial agents is able to shorten duration of illness the consequences are they able to resists against antimicrobial agents are increasing. The general aim for this study was to isolate and identification the presences of Shigella spp. in both traditional and commercial aquaculture farm. In order to achieve this, water sampling collection from I traditional pond and 2 aquaculture pond, isolated by XLD agar, differentiated by several biochemical tests and molecular analysis (polymerase chain reaction). Amplification of Shiga toxin (stx gene) was done to confirm the presences of pathogenic Shigella spp. in both type farms. Three cultures samples from each aquaculture farm were assessed with some appropriate antibiotics such as ampicillin, tetracycline, sulphonamides, erythromycin and sulfamethoxazole. Overall, the bacterial strain are able to resist the antibiotics.
format Final Year Project Report
author Stephanie Lenja, Sait
author_facet Stephanie Lenja, Sait
author_sort Stephanie Lenja, Sait
title Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
title_short Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
title_full Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
title_fullStr Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
title_full_unstemmed Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
title_sort isolation and characterization of shigella spp. from water in aquaculture farm
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35179/1/Stephanie%20Lenja%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35179/4/Stephanie%20Lenja%20ft.pdf
http://ir.unimas.my/id/eprint/35179/
_version_ 1808981486583414784
score 13.211869

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