Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm
Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri, Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However, their...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35179/1/Stephanie%20Lenja%2824pgs%29.pdf http://ir.unimas.my/id/eprint/35179/4/Stephanie%20Lenja%20ft.pdf http://ir.unimas.my/id/eprint/35179/ |
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| Summary: | Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal
cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri,
Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However,
their presences in aquaculture sector had become a concern, especially affect towards to human
health. Besides, treatment of shigellosis with appropriate antimicrobial agents is able to shorten
duration of illness the consequences are they able to resists against antimicrobial agents are
increasing. The general aim for this study was to isolate and identification the presences of Shigella
spp. in both traditional and commercial aquaculture farm. In order to achieve this, water sampling
collection from I traditional pond and 2 aquaculture pond, isolated by XLD agar, differentiated by
several biochemical tests and molecular analysis (polymerase chain reaction). Amplification of
Shiga toxin (stx gene) was done to confirm the presences of pathogenic Shigella spp. in both type
farms. Three cultures samples from each aquaculture farm were assessed with some appropriate
antibiotics such as ampicillin, tetracycline, sulphonamides, erythromycin and sulfamethoxazole.
Overall, the bacterial strain are able to resist the antibiotics. |
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