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Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities

Genetic variation of Piper nigrum L. depends on revelation of unique strains. Studies reported that amplified fragment length polymorphism (AFLP) technique have been used to detect the genetic diversity and examining genetic relationships in pepper berries (Piper nigrum L. ). Thus, the aim of thi...

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Main Author: Ameeratul Maryam, Zubair Khan
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS)
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/35174/1/Development%20of%20Biomarker%20or%20DNA%20Fingerprinting%20of%20Pepper%20Berries%20from%20Different%20Geographical%20Localities%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35174/4/Development%20of%20Biomarker%20..%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/35174/
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spelling my.unimas.ir.351742023-02-09T08:45:28Z http://ir.unimas.my/id/eprint/35174/ Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities Ameeratul Maryam, Zubair Khan Q Science (General) Genetic variation of Piper nigrum L. depends on revelation of unique strains. Studies reported that amplified fragment length polymorphism (AFLP) technique have been used to detect the genetic diversity and examining genetic relationships in pepper berries (Piper nigrum L. ). Thus, the aim of this research study is to develop biomarkers by extracting the fresh pepper berries DNA and to characterize the unique DNA between locations by looking at their banding patterns; Four geographical localities in Sarawak were chosen which are Serian, Padawan, Bako and Lundu. The pepper berries were collected, and a total of thirteen samples from two locations were successfully extracted from the pepper berries. The DNA from S 1 and Pl were chosen for selective polymerase chain reaction (PCR) with six AFLP primer combinations to identify the most suitable pairs of restriction endonucleases and selective primers. By using 3% agarose gel electrophoresis (AGE), result shows that five primer combinations produced unique bands whereas another one primer combination shows similar and shared fragments between Serian and Padawan samples. Universiti Malaysia Sarawak (UNIMAS) Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35174/1/Development%20of%20Biomarker%20or%20DNA%20Fingerprinting%20of%20Pepper%20Berries%20from%20Different%20Geographical%20Localities%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/35174/4/Development%20of%20Biomarker%20..%28fulltext%29.pdf Ameeratul Maryam, Zubair Khan Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Ameeratul Maryam, Zubair Khan
Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
description Genetic variation of Piper nigrum L. depends on revelation of unique strains. Studies reported that amplified fragment length polymorphism (AFLP) technique have been used to detect the genetic diversity and examining genetic relationships in pepper berries (Piper nigrum L. ). Thus, the aim of this research study is to develop biomarkers by extracting the fresh pepper berries DNA and to characterize the unique DNA between locations by looking at their banding patterns; Four geographical localities in Sarawak were chosen which are Serian, Padawan, Bako and Lundu. The pepper berries were collected, and a total of thirteen samples from two locations were successfully extracted from the pepper berries. The DNA from S 1 and Pl were chosen for selective polymerase chain reaction (PCR) with six AFLP primer combinations to identify the most suitable pairs of restriction endonucleases and selective primers. By using 3% agarose gel electrophoresis (AGE), result shows that five primer combinations produced unique bands whereas another one primer combination shows similar and shared fragments between Serian and Padawan samples.
format Final Year Project Report
author Ameeratul Maryam, Zubair Khan
author_facet Ameeratul Maryam, Zubair Khan
author_sort Ameeratul Maryam, Zubair Khan
title Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
title_short Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
title_full Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
title_fullStr Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
title_full_unstemmed Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
title_sort development of biomarker or dna fingerprinting of pepper berries from different geographical localities
publisher Universiti Malaysia Sarawak (UNIMAS)
url http://ir.unimas.my/id/eprint/35174/1/Development%20of%20Biomarker%20or%20DNA%20Fingerprinting%20of%20Pepper%20Berries%20from%20Different%20Geographical%20Localities%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35174/4/Development%20of%20Biomarker%20..%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/35174/
_version_ 1758582569976201216
score 13.211869

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