Development of Biomarker or DNA Fingerprinting of Pepper Berries from Different Geographical Localities
Genetic variation of Piper nigrum L. depends on revelation of unique strains. Studies reported that amplified fragment length polymorphism (AFLP) technique have been used to detect the genetic diversity and examining genetic relationships in pepper berries (Piper nigrum L. ). Thus, the aim of thi...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
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| Online Access: | http://ir.unimas.my/id/eprint/35174/1/Development%20of%20Biomarker%20or%20DNA%20Fingerprinting%20of%20Pepper%20Berries%20from%20Different%20Geographical%20Localities%2824pgs%29.pdf http://ir.unimas.my/id/eprint/35174/4/Development%20of%20Biomarker%20..%28fulltext%29.pdf http://ir.unimas.my/id/eprint/35174/ |
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| Summary: | Genetic variation of Piper nigrum L. depends on revelation of unique strains. Studies
reported that amplified fragment length polymorphism (AFLP) technique have been used to
detect the genetic diversity and examining genetic relationships in pepper berries (Piper
nigrum L. ). Thus, the aim of this research study is to develop biomarkers by extracting the
fresh pepper berries DNA and to characterize the unique DNA between locations by looking
at their banding patterns; Four geographical localities in Sarawak were chosen which are
Serian, Padawan, Bako and Lundu. The pepper berries were collected, and a total of thirteen
samples from two locations were successfully extracted from the pepper berries. The DNA
from S 1 and Pl were chosen for selective polymerase chain reaction (PCR) with six AFLP
primer combinations to identify the most suitable pairs of restriction endonucleases and
selective primers. By using 3% agarose gel electrophoresis (AGE), result shows that five
primer combinations produced unique bands whereas another one primer combination shows
similar and shared fragments between Serian and Padawan samples. |
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