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Reconstruction of msadh gene

Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relativel...

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Bibliographic Details
Main Author: Ho, Carl Miew
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf
http://ir.unimas.my/id/eprint/314/
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spelling my.unimas.ir.3142023-10-31T07:34:05Z http://ir.unimas.my/id/eprint/314/ Reconstruction of msadh gene Ho, Carl Miew Q Science (General) Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relatively low toxicity compound, ethanol. This vital response of plants has been proved in enhancing the potential for survival under hypoxic conditions with a low production of A TP and regeneration of NAD+· The objective of this study was to identify and verify the presence of AdhipET inserts within plasmid which is isolated from Metroxylon sagu via Restriction endonuclease and Polymerase Chain Reaction. In addition, reconstruction of the msAdh plasmid was carried out due to the frame shift of single nucleotide. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf Ho, Carl Miew (2011) Reconstruction of msadh gene. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Ho, Carl Miew
Reconstruction of msadh gene
description Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relatively low toxicity compound, ethanol. This vital response of plants has been proved in enhancing the potential for survival under hypoxic conditions with a low production of A TP and regeneration of NAD+· The objective of this study was to identify and verify the presence of AdhipET inserts within plasmid which is isolated from Metroxylon sagu via Restriction endonuclease and Polymerase Chain Reaction. In addition, reconstruction of the msAdh plasmid was carried out due to the frame shift of single nucleotide.
format Final Year Project Report
author Ho, Carl Miew
author_facet Ho, Carl Miew
author_sort Ho, Carl Miew
title Reconstruction of msadh gene
title_short Reconstruction of msadh gene
title_full Reconstruction of msadh gene
title_fullStr Reconstruction of msadh gene
title_full_unstemmed Reconstruction of msadh gene
title_sort reconstruction of msadh gene
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf
http://ir.unimas.my/id/eprint/314/
_version_ 1781710378957275136
score 13.211869

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