Reconstruction of msadh gene
Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relativel...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2011
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf http://ir.unimas.my/id/eprint/314/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which
normally occurs in most of plants. These genes are activated when under anaerobic stresses
by producing measurable level of alcohol dehydrogenase enzyme which is capable in
converting acetaldehyde into relatively low toxicity compound, ethanol. This vital response
of plants has been proved in enhancing the potential for survival under hypoxic conditions
with a low production of A TP and regeneration of NAD+· The objective of this study was
to identify and verify the presence of AdhipET inserts within plasmid which is isolated
from Metroxylon sagu via Restriction endonuclease and Polymerase Chain Reaction. In
addition, reconstruction of the msAdh plasmid was carried out due to the frame shift of
single nucleotide. |
|---|