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Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis

Tomistoma schlegelii or known as false gharial, is a freshwater crocodilian endemic to Malaysia and Indonesia. This species is currently affected with severe habitat loss as their numbers in the wild have been decreasing due to legal and illegal logging, urbanization, and unsustainable fishing activ...

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Main Author: Muhamad Farhan, Badri
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak(UNIMAS) 2015
Subjects:
Q Science (General)
QH426 Genetics
Online Access:http://ir.unimas.my/id/eprint/30324/1/Genetic%20Diversity%20of%20False%20Gharial%2024pgs.pdf
http://ir.unimas.my/id/eprint/30324/4/Genetic%20Diversity%20of%20False%20Gharial%20ft.pdf
http://ir.unimas.my/id/eprint/30324/
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spelling my.unimas.ir.303242023-08-21T08:34:37Z http://ir.unimas.my/id/eprint/30324/ Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis Muhamad Farhan, Badri Q Science (General) QH426 Genetics Tomistoma schlegelii or known as false gharial, is a freshwater crocodilian endemic to Malaysia and Indonesia. This species is currently affected with severe habitat loss as their numbers in the wild have been decreasing due to legal and illegal logging, urbanization, and unsustainable fishing activities. The genetic studies on gharials have been carried out but the genetic information regarding T. schlegelii is still lacking. Therefore, this study is designed to sequence the cytochrome b-control region (cyt b-CR) gene and to determine genetic diversity of T. schlegelii individuals in Sarawak. A total of 647 bp of cyt b-CR gene has been sequenced and it matched 99% sequences data of T. schlegelii (Accession No: HM593977). The new cyt b-CR sequence data has been deposited in the GenBank (Accession No: KR674082). Genetic divergence value between T. schlegelii in Asajaya and those from GenBank originated from Sarawak showed 1.1% distance. Both Neigbour-Joining and Maximum Parsimony phylogenetic trees agreed on the monophyletic status of T. schlegelii. Universiti Malaysia Sarawak(UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/30324/1/Genetic%20Diversity%20of%20False%20Gharial%2024pgs.pdf text en http://ir.unimas.my/id/eprint/30324/4/Genetic%20Diversity%20of%20False%20Gharial%20ft.pdf Muhamad Farhan, Badri (2015) Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
QH426 Genetics
spellingShingle Q Science (General)
QH426 Genetics
Muhamad Farhan, Badri
Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
description Tomistoma schlegelii or known as false gharial, is a freshwater crocodilian endemic to Malaysia and Indonesia. This species is currently affected with severe habitat loss as their numbers in the wild have been decreasing due to legal and illegal logging, urbanization, and unsustainable fishing activities. The genetic studies on gharials have been carried out but the genetic information regarding T. schlegelii is still lacking. Therefore, this study is designed to sequence the cytochrome b-control region (cyt b-CR) gene and to determine genetic diversity of T. schlegelii individuals in Sarawak. A total of 647 bp of cyt b-CR gene has been sequenced and it matched 99% sequences data of T. schlegelii (Accession No: HM593977). The new cyt b-CR sequence data has been deposited in the GenBank (Accession No: KR674082). Genetic divergence value between T. schlegelii in Asajaya and those from GenBank originated from Sarawak showed 1.1% distance. Both Neigbour-Joining and Maximum Parsimony phylogenetic trees agreed on the monophyletic status of T. schlegelii.
format Final Year Project Report
author Muhamad Farhan, Badri
author_facet Muhamad Farhan, Badri
author_sort Muhamad Farhan, Badri
title Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
title_short Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
title_full Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
title_fullStr Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
title_full_unstemmed Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
title_sort genetic diversity of false gharial tomistoma schlegelii based on cytochrome b-control region (cyt b-cr) gene analysis
publisher Universiti Malaysia Sarawak(UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/30324/1/Genetic%20Diversity%20of%20False%20Gharial%2024pgs.pdf
http://ir.unimas.my/id/eprint/30324/4/Genetic%20Diversity%20of%20False%20Gharial%20ft.pdf
http://ir.unimas.my/id/eprint/30324/
_version_ 1775627274877403136
score 13.211869

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