Genetic Diversity of False Gharial Tomistoma schlegelii based on Cytochrome b-Control Region (cyt b-CR) Gene Analysis
Tomistoma schlegelii or known as false gharial, is a freshwater crocodilian endemic to Malaysia and Indonesia. This species is currently affected with severe habitat loss as their numbers in the wild have been decreasing due to legal and illegal logging, urbanization, and unsustainable fishing activ...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak(UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/30324/1/Genetic%20Diversity%20of%20False%20Gharial%2024pgs.pdf http://ir.unimas.my/id/eprint/30324/4/Genetic%20Diversity%20of%20False%20Gharial%20ft.pdf http://ir.unimas.my/id/eprint/30324/ |
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| Summary: | Tomistoma schlegelii or known as false gharial, is a freshwater crocodilian endemic to Malaysia and Indonesia. This species is currently affected with severe habitat loss as their numbers in the wild have been decreasing due to legal and illegal logging, urbanization, and unsustainable fishing activities. The genetic studies on gharials have been carried out but the genetic information regarding T. schlegelii is still lacking. Therefore, this study is designed to sequence the cytochrome b-control region (cyt b-CR) gene and to determine genetic diversity of T. schlegelii individuals in Sarawak. A total of 647 bp of cyt b-CR gene has been sequenced and it matched 99% sequences data of T. schlegelii (Accession No: HM593977). The new cyt b-CR sequence data has been deposited in the GenBank (Accession No: KR674082). Genetic divergence value between T. schlegelii in Asajaya and those from GenBank originated from Sarawak showed 1.1% distance. Both Neigbour-Joining and Maximum Parsimony phylogenetic trees agreed on the monophyletic status of T. schlegelii. |
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