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Transformation of mungbean cotyledon by using agrobacterium tumefaciens

Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17 lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonala...

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Main Author: Syafiqah Diana, Binti Subandi.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2015
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://ir.unimas.my/id/eprint/27410/4/Syafiqah%20Diana%20Binti%20Subandi.pdf
http://ir.unimas.my/id/eprint/27410/
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spelling my.unimas.ir.274102024-01-31T08:01:23Z http://ir.unimas.my/id/eprint/27410/ Transformation of mungbean cotyledon by using agrobacterium tumefaciens Syafiqah Diana, Binti Subandi. Q Science (General) QH301 Biology Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17 lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonalax is (CAEA). These explants were co-culti va ted with Agrobaelerium lume(ociens in th e presence of acetosyringone. Binary vector harbouring luei/erase (LUe:') gene (pGPTV), was tested to examine its effec t on transformation efficiency. The cotyledons from treated and germinated seedlings anal ysed via polymerase chain reaction (peR). The res ult obtai ned fro m the comparison of the efficiency of cotyledon leaf (2-3 stages leaves) and cotyledon attached with embryonal axis were inconclusive . For further studies, the transformation efficiency needs 'to be in creased and also improve on the genomic DNA ex trac tion procedure. Universiti Malaysia Sarawak (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/27410/4/Syafiqah%20Diana%20Binti%20Subandi.pdf Syafiqah Diana, Binti Subandi. (2015) Transformation of mungbean cotyledon by using agrobacterium tumefaciens. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Syafiqah Diana, Binti Subandi.
Transformation of mungbean cotyledon by using agrobacterium tumefaciens
description Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17 lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonalax is (CAEA). These explants were co-culti va ted with Agrobaelerium lume(ociens in th e presence of acetosyringone. Binary vector harbouring luei/erase (LUe:') gene (pGPTV), was tested to examine its effec t on transformation efficiency. The cotyledons from treated and germinated seedlings anal ysed via polymerase chain reaction (peR). The res ult obtai ned fro m the comparison of the efficiency of cotyledon leaf (2-3 stages leaves) and cotyledon attached with embryonal axis were inconclusive . For further studies, the transformation efficiency needs 'to be in creased and also improve on the genomic DNA ex trac tion procedure.
format Final Year Project Report
author Syafiqah Diana, Binti Subandi.
author_facet Syafiqah Diana, Binti Subandi.
author_sort Syafiqah Diana, Binti Subandi.
title Transformation of mungbean cotyledon by using agrobacterium tumefaciens
title_short Transformation of mungbean cotyledon by using agrobacterium tumefaciens
title_full Transformation of mungbean cotyledon by using agrobacterium tumefaciens
title_fullStr Transformation of mungbean cotyledon by using agrobacterium tumefaciens
title_full_unstemmed Transformation of mungbean cotyledon by using agrobacterium tumefaciens
title_sort transformation of mungbean cotyledon by using agrobacterium tumefaciens
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/27410/4/Syafiqah%20Diana%20Binti%20Subandi.pdf
http://ir.unimas.my/id/eprint/27410/
_version_ 1789945462245031936
score 13.211869

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