Transformation of mungbean cotyledon by using agrobacterium tumefaciens
Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17 lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonala...
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/27410/4/Syafiqah%20Diana%20Binti%20Subandi.pdf http://ir.unimas.my/id/eprint/27410/ |
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| Summary: | Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17
lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonalax is (CAEA). These explants were co-culti va ted with Agrobaelerium lume(ociens in th e presence of acetosyringone. Binary vector harbouring
luei/erase (LUe:') gene (pGPTV), was tested to examine its effec t on transformation efficiency. The cotyledons from treated and germinated seedlings anal ysed via polymerase chain reaction (peR). The res ult obtai ned fro m the comparison of the efficiency of cotyledon leaf (2-3 stages leaves) and cotyledon attached with embryonal axis were inconclusive . For further studies, the
transformation efficiency needs 'to be in creased and also improve on the genomic DNA ex trac tion procedure. |
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