Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water

Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of chloramphenicol has induced the formation of chloramphenicol-resistant bact...

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Main Author: Cheng, Wei Kang.
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/24101/2/Distribution%20of%20chloramphenicol%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/24101/3/Distribution%20of%20chloramphenicol%20fulltext.pdf
http://ir.unimas.my/id/eprint/24101/
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spelling my.unimas.ir.241012023-01-27T08:20:35Z http://ir.unimas.my/id/eprint/24101/ Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water Cheng, Wei Kang. S Agriculture (General) Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of chloramphenicol has induced the formation of chloramphenicol-resistant bacteria including the Escherichia coli. These chloramphenicol-rcsistant genes can be found in E. coli in rivers near aquaculture farm. The objective of this research i to determine the distribution of chloramphenicol-resistant gene in E. coli isolated from aquacultural environment and environmental water. In this project 10 isolated bacteria from water samples were analyzed for chloramphcnicol antibiotic resistant genes (cat I, cat II, cat III and cat IV genes) by using mUltiplex PCR. All E. coli i olates are found to contain two different cat genes. All 10 isolates are found to have cat II genes. 5 isolates have cat IV genes, 3 isolates have cat IV genes and another 2 have cat I genes. This study can help in studying the significance in antibiotic resistant gene and how such differences contribute to the survival of the bacteria. The result from this research can also help inform the farmers to the risk of using chloramphenicol as antibiotic. The usage of chloramphenicol can cause the proliferation of chloramphenicol resistant bacteria and may spread to environmental water through water disposal from aquaculture farm. Universiti Malaysia Sarawak (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/24101/2/Distribution%20of%20chloramphenicol%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/24101/3/Distribution%20of%20chloramphenicol%20fulltext.pdf Cheng, Wei Kang. (2015) Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Cheng, Wei Kang.
Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
description Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of chloramphenicol has induced the formation of chloramphenicol-resistant bacteria including the Escherichia coli. These chloramphenicol-rcsistant genes can be found in E. coli in rivers near aquaculture farm. The objective of this research i to determine the distribution of chloramphenicol-resistant gene in E. coli isolated from aquacultural environment and environmental water. In this project 10 isolated bacteria from water samples were analyzed for chloramphcnicol antibiotic resistant genes (cat I, cat II, cat III and cat IV genes) by using mUltiplex PCR. All E. coli i olates are found to contain two different cat genes. All 10 isolates are found to have cat II genes. 5 isolates have cat IV genes, 3 isolates have cat IV genes and another 2 have cat I genes. This study can help in studying the significance in antibiotic resistant gene and how such differences contribute to the survival of the bacteria. The result from this research can also help inform the farmers to the risk of using chloramphenicol as antibiotic. The usage of chloramphenicol can cause the proliferation of chloramphenicol resistant bacteria and may spread to environmental water through water disposal from aquaculture farm.
format Final Year Project Report
author Cheng, Wei Kang.
author_facet Cheng, Wei Kang.
author_sort Cheng, Wei Kang.
title Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
title_short Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
title_full Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
title_fullStr Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
title_full_unstemmed Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
title_sort distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/24101/2/Distribution%20of%20chloramphenicol%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/24101/3/Distribution%20of%20chloramphenicol%20fulltext.pdf
http://ir.unimas.my/id/eprint/24101/
_version_ 1756688305293885440
score 13.211869