Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water
Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of chloramphenicol has induced the formation of chloramphenicol-resistant bact...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/24101/2/Distribution%20of%20chloramphenicol%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/24101/3/Distribution%20of%20chloramphenicol%20fulltext.pdf http://ir.unimas.my/id/eprint/24101/ |
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| Summary: | Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic
chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of
chloramphenicol has induced the formation of chloramphenicol-resistant bacteria including the Escherichia coli.
These chloramphenicol-rcsistant genes can be found in E. coli in rivers near aquaculture farm. The objective of
this research i to determine the distribution of chloramphenicol-resistant gene in E. coli isolated from
aquacultural environment and environmental water. In this project 10 isolated bacteria from water samples were
analyzed for chloramphcnicol antibiotic resistant genes (cat I, cat II, cat III and cat IV genes) by using mUltiplex
PCR. All E. coli i olates are found to contain two different cat genes. All 10 isolates are found to have cat II
genes. 5 isolates have cat IV genes, 3 isolates have cat IV genes and another 2 have cat I genes. This study can
help in studying the significance in antibiotic resistant gene and how such differences contribute to the survival of the bacteria. The result from this research can also help inform the farmers to the risk of using chloramphenicol as antibiotic. The usage of chloramphenicol can cause the proliferation of chloramphenicol resistant bacteria and may spread to environmental water through water disposal from aquaculture farm. |
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