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Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak

Magnaporl!7e OIyzae is a fungal pathogen responsible for rice blast disease all over the globe. Resistance or susceptibility of a rice cultivar results from the interaction between R (resistance) gene of the plant and Avr (avirulence) gene of the pathogen. While more research focus on the functions...

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Main Author: Jee, Mui Sie
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2016
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/23790/1/Jee%20Mui%20Sie.pdf
http://ir.unimas.my/id/eprint/23790/4/Jee%20Mui%20Sie%20-%20full.pdf
http://ir.unimas.my/id/eprint/23790/
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spelling my.unimas.ir.237902023-06-27T08:09:12Z http://ir.unimas.my/id/eprint/23790/ Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak Jee, Mui Sie S Agriculture (General) Magnaporl!7e OIyzae is a fungal pathogen responsible for rice blast disease all over the globe. Resistance or susceptibility of a rice cultivar results from the interaction between R (resistance) gene of the plant and Avr (avirulence) gene of the pathogen. While more research focus on the functions ofR gene, it should be aware that Avr gene also plays an important role in plant disease control. In this study, full-length cod ing sequence of AvrPizI gene was studied by initial fungal species verification using uni versa l primer pair ITS 1IITS4 followed by AvrPiz-1 gene isolation by direct peR using specific primer pair. Then, the amp lified gene was subjected to cloning prior to subsequent sequencing. The presence of AvrPiz-1 gene with an open reading frame of 326 bp encoding for 108 amino acid residues was detected and confirmed in M. oryzae 7', a Sarawak isolate . From the sequencing output, three uncharacterized nucleotide mutations were found across pairwise sequence alignment with an avirulent strain. Thus, the functional sign ificance of these sequence variations on the avirulent phenotype of AvrPi::-1 gene found ha s yet to be investigated. Not forgotten, several high occurrence motifs usually involved in signal transduction pathways were detected across the nucleotide translated protein sequence. This suggests the possible role of the avirulence protein in host-pathogen interaction. Most importantly, a novel motif was predicted across the protein sequence of five avirulence proteins (AvrPiz-l, AvrPi9, A VR -Pia, AVR-Pii, and AvrPib) invo lving in the triggering of plant defe nse system. This imply that the proteins might share some degree of unknown similarity which might un veil the mechanism of funga l pathogenesis and therefore useful for the study in rice blast control. Universiti Malaysia Sarawak, (UNIMAS) 2016 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/23790/1/Jee%20Mui%20Sie.pdf text en http://ir.unimas.my/id/eprint/23790/4/Jee%20Mui%20Sie%20-%20full.pdf Jee, Mui Sie (2016) Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Jee, Mui Sie
Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
description Magnaporl!7e OIyzae is a fungal pathogen responsible for rice blast disease all over the globe. Resistance or susceptibility of a rice cultivar results from the interaction between R (resistance) gene of the plant and Avr (avirulence) gene of the pathogen. While more research focus on the functions ofR gene, it should be aware that Avr gene also plays an important role in plant disease control. In this study, full-length cod ing sequence of AvrPizI gene was studied by initial fungal species verification using uni versa l primer pair ITS 1IITS4 followed by AvrPiz-1 gene isolation by direct peR using specific primer pair. Then, the amp lified gene was subjected to cloning prior to subsequent sequencing. The presence of AvrPiz-1 gene with an open reading frame of 326 bp encoding for 108 amino acid residues was detected and confirmed in M. oryzae 7', a Sarawak isolate . From the sequencing output, three uncharacterized nucleotide mutations were found across pairwise sequence alignment with an avirulent strain. Thus, the functional sign ificance of these sequence variations on the avirulent phenotype of AvrPi::-1 gene found ha s yet to be investigated. Not forgotten, several high occurrence motifs usually involved in signal transduction pathways were detected across the nucleotide translated protein sequence. This suggests the possible role of the avirulence protein in host-pathogen interaction. Most importantly, a novel motif was predicted across the protein sequence of five avirulence proteins (AvrPiz-l, AvrPi9, A VR -Pia, AVR-Pii, and AvrPib) invo lving in the triggering of plant defe nse system. This imply that the proteins might share some degree of unknown similarity which might un veil the mechanism of funga l pathogenesis and therefore useful for the study in rice blast control.
format Final Year Project Report
author Jee, Mui Sie
author_facet Jee, Mui Sie
author_sort Jee, Mui Sie
title Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
title_short Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
title_full Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
title_fullStr Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
title_full_unstemmed Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
title_sort cloning and sequence analaysis of avrpiz-t gene from magnaporthe oryzae isolates from sarawak
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2016
url http://ir.unimas.my/id/eprint/23790/1/Jee%20Mui%20Sie.pdf
http://ir.unimas.my/id/eprint/23790/4/Jee%20Mui%20Sie%20-%20full.pdf
http://ir.unimas.my/id/eprint/23790/
_version_ 1769847637335343104
score 13.211869

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