Cloning and sequence analaysis of AvrPiz-t gene from Magnaporthe oryzae isolates from Sarawak
Magnaporl!7e OIyzae is a fungal pathogen responsible for rice blast disease all over the globe. Resistance or susceptibility of a rice cultivar results from the interaction between R (resistance) gene of the plant and Avr (avirulence) gene of the pathogen. While more research focus on the functions...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2016
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/23790/1/Jee%20Mui%20Sie.pdf http://ir.unimas.my/id/eprint/23790/4/Jee%20Mui%20Sie%20-%20full.pdf http://ir.unimas.my/id/eprint/23790/ |
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| Summary: | Magnaporl!7e OIyzae is a fungal pathogen responsible for rice blast disease all over the globe. Resistance or susceptibility of a rice cultivar results from the interaction between R (resistance) gene of the plant and Avr (avirulence) gene of the pathogen. While more
research focus on the functions ofR gene, it should be aware that Avr gene also plays an
important role in plant disease control. In this study, full-length cod ing sequence of AvrPizI gene was studied by initial fungal species verification using uni versa l primer pair
ITS 1IITS4 followed by AvrPiz-1 gene isolation by direct peR using specific primer pair.
Then, the amp lified gene was subjected to cloning prior to subsequent sequencing. The
presence of AvrPiz-1 gene with an open reading frame of 326 bp encoding for 108 amino
acid residues was detected and confirmed in M. oryzae 7', a Sarawak isolate . From the
sequencing output, three uncharacterized nucleotide mutations were found across pairwise
sequence alignment with an avirulent strain. Thus, the functional sign ificance of these
sequence variations on the avirulent phenotype of AvrPi::-1 gene found ha s yet to be
investigated. Not forgotten, several high occurrence motifs usually involved in signal
transduction pathways were detected across the nucleotide translated protein sequence.
This suggests the possible role of the avirulence protein in host-pathogen interaction. Most
importantly, a novel motif was predicted across the protein sequence of five avirulence
proteins (AvrPiz-l, AvrPi9, A VR -Pia, AVR-Pii, and AvrPib) invo lving in the triggering of
plant defe nse system. This imply that the proteins might share some degree of unknown
similarity which might un veil the mechanism of funga l pathogenesis and therefore useful
for the study in rice blast control. |
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