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Molecular detection and protein analysis of different type of frozen food product for halal authentication

Muslim consumers need to consume `Halal' food in order to follow Syariah Law. However, the detection of non halal source in the frozen food products may be occurred due to the adulteration in the food. Halal sausage, halal meatball, non halal sausage and non halal meatball were tested using Pol...

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Bibliographic Details
Main Author: Hasyimah, Harun
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2014
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/23741/2/Hasyimah%20ft.pdf
http://ir.unimas.my/id/eprint/23741/
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spelling my.unimas.ir.237412023-10-19T03:15:47Z http://ir.unimas.my/id/eprint/23741/ Molecular detection and protein analysis of different type of frozen food product for halal authentication Hasyimah, Harun Q Science (General) QR Microbiology Muslim consumers need to consume `Halal' food in order to follow Syariah Law. However, the detection of non halal source in the frozen food products may be occurred due to the adulteration in the food. Halal sausage, halal meatball, non halal sausage and non halal meatball were tested using Polymerase Chain Reaction (PCR) amplification of mitochondrial DNA. Three types of primers were used which were mitochondria DNA of cytochrome b (two types of primers) and mitochondria 12s rRNA (mt-12s rDNA) (one type of primer). The PCR amplification products from cyt b gene and mt- 12s rDNA were unsuccessful to amplify as there were only primer-dimer band formed on the agarose gel. Protein of halal sausage, halal meatball, non halal sausage and non halal meatball were extracted and Bovine Serum Albumin (BSA) standard curve was plotted based on Bradford protein concentration assay. Protein concentrations in all four samples were calculated and Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS PAGE) was conducted for protein analysis on all of four samples. There were difference in the clear and prominent bands on all of four samples. Universiti Malaysia Sarawak (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/23741/2/Hasyimah%20ft.pdf Hasyimah, Harun (2014) Molecular detection and protein analysis of different type of frozen food product for halal authentication. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Hasyimah, Harun
Molecular detection and protein analysis of different type of frozen food product for halal authentication
description Muslim consumers need to consume `Halal' food in order to follow Syariah Law. However, the detection of non halal source in the frozen food products may be occurred due to the adulteration in the food. Halal sausage, halal meatball, non halal sausage and non halal meatball were tested using Polymerase Chain Reaction (PCR) amplification of mitochondrial DNA. Three types of primers were used which were mitochondria DNA of cytochrome b (two types of primers) and mitochondria 12s rRNA (mt-12s rDNA) (one type of primer). The PCR amplification products from cyt b gene and mt- 12s rDNA were unsuccessful to amplify as there were only primer-dimer band formed on the agarose gel. Protein of halal sausage, halal meatball, non halal sausage and non halal meatball were extracted and Bovine Serum Albumin (BSA) standard curve was plotted based on Bradford protein concentration assay. Protein concentrations in all four samples were calculated and Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS PAGE) was conducted for protein analysis on all of four samples. There were difference in the clear and prominent bands on all of four samples.
format Final Year Project Report
author Hasyimah, Harun
author_facet Hasyimah, Harun
author_sort Hasyimah, Harun
title Molecular detection and protein analysis of different type of frozen food product for halal authentication
title_short Molecular detection and protein analysis of different type of frozen food product for halal authentication
title_full Molecular detection and protein analysis of different type of frozen food product for halal authentication
title_fullStr Molecular detection and protein analysis of different type of frozen food product for halal authentication
title_full_unstemmed Molecular detection and protein analysis of different type of frozen food product for halal authentication
title_sort molecular detection and protein analysis of different type of frozen food product for halal authentication
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/23741/2/Hasyimah%20ft.pdf
http://ir.unimas.my/id/eprint/23741/
_version_ 1781710283353358336
score 13.211869

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