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Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002

The effect of solid state fermentation (SSF) parameters on the production of extracellular a-amylase and celulase (endoglucanases) by Bacilllls amyloliqueJaciens UMAS 1002 using sago pith residue "ham pas" in shaker flasks was investigated in this study. The appropriate incubation period...

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Main Author: Faisal Ali, Anwarali Khan
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS) 2004
Subjects:
QK Botany
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/1332/1/Faisal%20Ali.pdf
http://ir.unimas.my/id/eprint/1332/2/Faisal%20Ali.pdf
http://ir.unimas.my/id/eprint/1332/
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spelling my.unimas.ir.13322023-08-30T04:51:15Z http://ir.unimas.my/id/eprint/1332/ Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002 Faisal Ali, Anwarali Khan QK Botany QR Microbiology The effect of solid state fermentation (SSF) parameters on the production of extracellular a-amylase and celulase (endoglucanases) by Bacilllls amyloliqueJaciens UMAS 1002 using sago pith residue "ham pas" in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, " hampas" concentration and additive substrate effect were optimized for enzyme production. The activity of a-amylase and cellulase was at 14.19 I.U.lml and 13.15 I.U.lml respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v "hampas" in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 hours with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for a-amylase and cellulase respectively. Enzyme activity was 2.8 (10.80 I.U.lml) and 3.2 (9.38 I.U.lml) fold higher for a-amylase and cellulase respectively when 1 % w/v soluble starch was applied as additive sub !rates with 0.5% "hampas" . However in optimal media that consist of 4%w/v of "hampas", addition of 1 % w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of a-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, dissolved oxygen (DO), and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too. Universiti Malaysia Sarawak (UNIMAS) 2004 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/1332/1/Faisal%20Ali.pdf text en http://ir.unimas.my/id/eprint/1332/2/Faisal%20Ali.pdf Faisal Ali, Anwarali Khan (2004) Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QK Botany
QR Microbiology
spellingShingle QK Botany
QR Microbiology
Faisal Ali, Anwarali Khan
Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
description The effect of solid state fermentation (SSF) parameters on the production of extracellular a-amylase and celulase (endoglucanases) by Bacilllls amyloliqueJaciens UMAS 1002 using sago pith residue "ham pas" in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, " hampas" concentration and additive substrate effect were optimized for enzyme production. The activity of a-amylase and cellulase was at 14.19 I.U.lml and 13.15 I.U.lml respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v "hampas" in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 hours with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for a-amylase and cellulase respectively. Enzyme activity was 2.8 (10.80 I.U.lml) and 3.2 (9.38 I.U.lml) fold higher for a-amylase and cellulase respectively when 1 % w/v soluble starch was applied as additive sub !rates with 0.5% "hampas" . However in optimal media that consist of 4%w/v of "hampas", addition of 1 % w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of a-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, dissolved oxygen (DO), and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too.
format Final Year Project Report
author Faisal Ali, Anwarali Khan
author_facet Faisal Ali, Anwarali Khan
author_sort Faisal Ali, Anwarali Khan
title Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
title_short Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
title_full Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
title_fullStr Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
title_full_unstemmed Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
title_sort enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens umas 1002
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2004
url http://ir.unimas.my/id/eprint/1332/1/Faisal%20Ali.pdf
http://ir.unimas.my/id/eprint/1332/2/Faisal%20Ali.pdf
http://ir.unimas.my/id/eprint/1332/
_version_ 1776251345957814272
score 13.211869

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