Antioxidant activities and polyphenolics of various solvent extracts of red seaweed, Gracilaria changii
This study determined the levels of polyphenols, flavonoids, carotenoids and antioxidant activity of the edible red seaweed, Gracilaria changii. Freeze-dried G. changii powder was extracted using five solvent systems: 80 % ethanol, 80 % methanol, 80 % acetone, ethyl acetate and water. The antioxida...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Netherlands
2015
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/13019/1/Antioxidant.pdf http://ir.unimas.my/id/eprint/13019/ http://link.springer.com/article/10.1007/s10811-014-0493-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study determined the levels of polyphenols,
flavonoids, carotenoids and antioxidant activity of the edible red seaweed, Gracilaria changii. Freeze-dried G. changii powder was extracted using five solvent systems: 80 % ethanol, 80 % methanol, 80 % acetone, ethyl acetate and water. The antioxidant activity of the extracts was measured using three assays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power (FRAP) and β-carotene bleaching assays (BCB). The total phenolics (TPC) and total flavonoids content (TFC) and the total carotenoids content (TCC) were also determined. Ethanol extract had the highest
yield followed by methanol > water > acetone > ethyl
acetate extract. The radical scavenging effects of all the
extracts showing dose-dependency properties with the ethyl
acetate extract of G. changii exhibiting the highest
radical scavenging effect (EC50 of 0.51±0.09 mg mL−1),
while the water extract had the lowest scavenging effect of
7.80±0.57 mg mL−1. Ferric reducing power was in the
following descending order: ethyl acetate > ethanol >
methanol > acetone > water. TPC was highest in the ethyl
acetate extract (21.57±2.58 mg PGE g−1) and least in the
water extract (6.06±0.52 mg PGE g−1). The TFC of
G. changii was in the range of 18.97±0.89–200.87±
3.61 mg RE g−1. The results revealed the effect of
different extracting solvents in altering the antioxidant
potential of G.changii, and ethyl acetate was identified
as the most efficient solvent for extracting bioactive compounds from this red seaweed. |
|---|