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Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed...

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Main Author: Sing, Tung Teng
Format: E-Article
Language:English
Published: Springer Japan 2015
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10719/
http://link.springer.com/article/10.1007%2Fs12562-015-0902-7
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spelling my.unimas.ir.107192016-10-21T06:42:07Z http://ir.unimas.my/id/eprint/10719/ Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA Sing, Tung Teng SH Aquaculture. Fisheries. Angling An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1– 8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding. Springer Japan 2015 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf Sing, Tung Teng (2015) Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fisheries Science, 2015 (81). pp. 831-838. ISSN 1444-2906 http://link.springer.com/article/10.1007%2Fs12562-015-0902-7 DOI : 10.1007/s12562-015-0902-7
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Sing, Tung Teng
Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
description An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1– 8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.
format E-Article
author Sing, Tung Teng
author_facet Sing, Tung Teng
author_sort Sing, Tung Teng
title Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
title_short Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
title_full Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
title_fullStr Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
title_full_unstemmed Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
title_sort pseudo‑nitzschia species (bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to lsu rdna
publisher Springer Japan
publishDate 2015
url http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10719/
http://link.springer.com/article/10.1007%2Fs12562-015-0902-7
_version_ 1644511038423433216
score 13.244404

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