Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed...
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| Format: | E-Article |
| Language: | English |
| Published: |
Springer Japan
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/10719/ http://link.springer.com/article/10.1007%2Fs12562-015-0902-7 |
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| Summary: | An attempt was made to infer the phylogeny
of Pseudo-nitzschia species by using the mitochondrialencoded
gene, cytochrome c oxidase subunit I (cox1),
and comparing it with the nuclear-encoded large subunit
ribosomal DNA (LSU rDNA). A pair of primers targeting
Pseudo-nitzschia cox1 was designed in silico and used to
infer the molecular phylogeny of Pseudo-nitzschia. The
primer pair was tested using genomic DNAs isolated from
six species of Pseudo-nitzschia from Malaysia. The phylogenetic
inference of cox1 was then compared to the LSU
rDNA phylogeny. Phylogenetic reconstructions of both
data sets revealed monophyly of Pseudo-nitzschia species
complexes. The range of genetic divergences among
Pseudo-nitzschia species were higher in the cox1 data set
(3.5–20.4 %) compared to the LSU rDNA data set (0.1–
8.8 %). The present study suggests that high genetic divergence
in cox1 of Pseudo-nitzschia species could be a useful
genetic marker for DNA bar coding. |
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