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Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak

Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for...

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Main Authors: Ho Wei, Seng, Liew, K.S., A.B., Elias, F.B., Fedrick, M.A., Mohamad Khairil, Julaihi, Abdullah, Phui, Seng Loi
Format: E-Article
Language:English
Published: Pelagia Research Library 2014
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10229/
https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC
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spelling my.unimas.ir.102292016-01-18T06:56:25Z http://ir.unimas.my/id/eprint/10229/ Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak Ho Wei, Seng Liew, K.S. A.B., Elias F.B., Fedrick M.A., Mohamad Khairil Julaihi, Abdullah Phui, Seng Loi SB Plant culture Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for large-scale planted forest development in Sarawak. Therefore, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Intersimple sequence repeats (ISSR) markers were used in this study to determine the genetic diversity of kelampayan in three progeny trial blocks at the Landeh Nature Reserve, Semengok, Sarawak. The seeds were collected from the selected mother trees located at the Pasai Bon, Niah and Lawas seed production areas (SPAs) in Sarawak. Three ISSR primers, namely (GTG)6, (AG)10 and (AC)10, that yielded reproducible, informative and scorable fragments were chosen for ISSR analysis. A total of 64 loci were generated of which 45.3–74.6% of the loci were identified as polymorphic bands with the size ranging from 500 bp to 2 kb among 247 kelampayan progenies selected in the present study. Molecular diversity based on Shannon’s diversity indices (I) among 247 trees ranged from 0.268 to 0.350. In general, the kelampayan trees in the three progeny trial blocks exhibited a high level of molecular diversity and DNA polymorphism compared with its natural populations. This preliminary information will form the base for kelampayan tree improvement and conservation programmes. Pelagia Research Library 2014 E-Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf Ho Wei, Seng and Liew, K.S. and A.B., Elias and F.B., Fedrick and M.A., Mohamad Khairil and Julaihi, Abdullah and Phui, Seng Loi (2014) Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak. Advances in Applied Science Research, 5 (3). pp. 458-463. ISSN 0976-8610 https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Ho Wei, Seng
Liew, K.S.
A.B., Elias
F.B., Fedrick
M.A., Mohamad Khairil
Julaihi, Abdullah
Phui, Seng Loi
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
description Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for large-scale planted forest development in Sarawak. Therefore, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Intersimple sequence repeats (ISSR) markers were used in this study to determine the genetic diversity of kelampayan in three progeny trial blocks at the Landeh Nature Reserve, Semengok, Sarawak. The seeds were collected from the selected mother trees located at the Pasai Bon, Niah and Lawas seed production areas (SPAs) in Sarawak. Three ISSR primers, namely (GTG)6, (AG)10 and (AC)10, that yielded reproducible, informative and scorable fragments were chosen for ISSR analysis. A total of 64 loci were generated of which 45.3–74.6% of the loci were identified as polymorphic bands with the size ranging from 500 bp to 2 kb among 247 kelampayan progenies selected in the present study. Molecular diversity based on Shannon’s diversity indices (I) among 247 trees ranged from 0.268 to 0.350. In general, the kelampayan trees in the three progeny trial blocks exhibited a high level of molecular diversity and DNA polymorphism compared with its natural populations. This preliminary information will form the base for kelampayan tree improvement and conservation programmes.
format E-Article
author Ho Wei, Seng
Liew, K.S.
A.B., Elias
F.B., Fedrick
M.A., Mohamad Khairil
Julaihi, Abdullah
Phui, Seng Loi
author_facet Ho Wei, Seng
Liew, K.S.
A.B., Elias
F.B., Fedrick
M.A., Mohamad Khairil
Julaihi, Abdullah
Phui, Seng Loi
author_sort Ho Wei, Seng
title Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_short Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_full Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_fullStr Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_full_unstemmed Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_sort genetic diversity of kelampayan using dominant dna markers based on inter-simple sequence repeats (issrs) in sarawak
publisher Pelagia Research Library
publishDate 2014
url http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10229/
https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC
_version_ 1644510912191660032
score 13.211869

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