Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for...
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2014
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| オンライン･アクセス: | http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/10229/ https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC |
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my.unimas.ir.102292016-01-18T06:56:25Z http://ir.unimas.my/id/eprint/10229/ Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak Ho Wei, Seng Liew, K.S. A.B., Elias F.B., Fedrick M.A., Mohamad Khairil Julaihi, Abdullah Phui, Seng Loi SB Plant culture Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for large-scale planted forest development in Sarawak. Therefore, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Intersimple sequence repeats (ISSR) markers were used in this study to determine the genetic diversity of kelampayan in three progeny trial blocks at the Landeh Nature Reserve, Semengok, Sarawak. The seeds were collected from the selected mother trees located at the Pasai Bon, Niah and Lawas seed production areas (SPAs) in Sarawak. Three ISSR primers, namely (GTG)6, (AG)10 and (AC)10, that yielded reproducible, informative and scorable fragments were chosen for ISSR analysis. A total of 64 loci were generated of which 45.3–74.6% of the loci were identified as polymorphic bands with the size ranging from 500 bp to 2 kb among 247 kelampayan progenies selected in the present study. Molecular diversity based on Shannon’s diversity indices (I) among 247 trees ranged from 0.268 to 0.350. In general, the kelampayan trees in the three progeny trial blocks exhibited a high level of molecular diversity and DNA polymorphism compared with its natural populations. This preliminary information will form the base for kelampayan tree improvement and conservation programmes. Pelagia Research Library 2014 E-Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf Ho Wei, Seng and Liew, K.S. and A.B., Elias and F.B., Fedrick and M.A., Mohamad Khairil and Julaihi, Abdullah and Phui, Seng Loi (2014) Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak. Advances in Applied Science Research, 5 (3). pp. 458-463. ISSN 0976-8610 https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC |
| institution |
Universiti Malaysia Sarawak |
| building |
Centre for Academic Information Services (CAIS) |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaysia Sarawak |
| content_source |
UNIMAS Institutional Repository |
| url_provider |
http://ir.unimas.my/ |
| language |
English |
| topic |
SB Plant culture |
| spellingShingle |
SB Plant culture Ho Wei, Seng Liew, K.S. A.B., Elias F.B., Fedrick M.A., Mohamad Khairil Julaihi, Abdullah Phui, Seng Loi Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| description |
Neolamarckia cadamba (Roxb.) Bosser, or locally known as
kelampayan, is a fast-growing timber species which produces one of
the best sources of raw material for the plywood industry and also for the
pulp and paper industry. It has been selected as one of the promising
plantation tree species for large-scale planted forest development in
Sarawak. Therefore, the molecular characterization of this indigenous
tropical tree species is needed to maintain its high quality. Intersimple
sequence repeats (ISSR) markers were used in this study
to determine the genetic diversity of kelampayan in three progeny
trial blocks at the Landeh Nature Reserve, Semengok, Sarawak. The
seeds were collected from the selected mother trees located at the
Pasai Bon, Niah and Lawas seed production areas (SPAs) in Sarawak.
Three ISSR primers, namely (GTG)6, (AG)10 and (AC)10, that yielded
reproducible, informative and scorable fragments were chosen for
ISSR analysis. A total of 64 loci were generated of which 45.3–74.6%
of the loci were identified as polymorphic bands with the size ranging
from 500 bp to 2 kb among 247 kelampayan progenies selected in
the present study. Molecular diversity based on Shannon’s diversity
indices (I) among 247 trees ranged from 0.268 to 0.350. In general,
the kelampayan trees in the three progeny trial blocks exhibited a high
level of molecular diversity and DNA polymorphism compared with its
natural populations. This preliminary information will form the base
for kelampayan tree improvement and conservation programmes. |
| format |
E-Article |
| author |
Ho Wei, Seng Liew, K.S. A.B., Elias F.B., Fedrick M.A., Mohamad Khairil Julaihi, Abdullah Phui, Seng Loi |
| author_facet |
Ho Wei, Seng Liew, K.S. A.B., Elias F.B., Fedrick M.A., Mohamad Khairil Julaihi, Abdullah Phui, Seng Loi |
| author_sort |
Ho Wei, Seng |
| title |
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| title_short |
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| title_full |
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| title_fullStr |
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| title_full_unstemmed |
Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak |
| title_sort |
genetic diversity of kelampayan using dominant dna markers based on inter-simple sequence repeats (issrs) in sarawak |
| publisher |
Pelagia Research Library |
| publishDate |
2014 |
| url |
http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/10229/ https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC |
| _version_ |
1644510912191660032 |
| score |
13.250246 |