Genetic diversity of Kelampayan using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
Neolamarckia cadamba (Roxb.) Bosser, or locally known as kelampayan, is a fast-growing timber species which produces one of the best sources of raw material for the plywood industry and also for the pulp and paper industry. It has been selected as one of the promising plantation tree species for...
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| Main Authors: | , , , , , , |
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| Format: | E-Article |
| Language: | English |
| Published: |
Pelagia Research Library
2014
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10229/1/Genetic%20diversity%20of%20Kelampayan%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/10229/ https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:LhH-TYMQEocC |
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| Summary: | Neolamarckia cadamba (Roxb.) Bosser, or locally known as
kelampayan, is a fast-growing timber species which produces one of
the best sources of raw material for the plywood industry and also for the
pulp and paper industry. It has been selected as one of the promising
plantation tree species for large-scale planted forest development in
Sarawak. Therefore, the molecular characterization of this indigenous
tropical tree species is needed to maintain its high quality. Intersimple
sequence repeats (ISSR) markers were used in this study
to determine the genetic diversity of kelampayan in three progeny
trial blocks at the Landeh Nature Reserve, Semengok, Sarawak. The
seeds were collected from the selected mother trees located at the
Pasai Bon, Niah and Lawas seed production areas (SPAs) in Sarawak.
Three ISSR primers, namely (GTG)6, (AG)10 and (AC)10, that yielded
reproducible, informative and scorable fragments were chosen for
ISSR analysis. A total of 64 loci were generated of which 45.3–74.6%
of the loci were identified as polymorphic bands with the size ranging
from 500 bp to 2 kb among 247 kelampayan progenies selected in
the present study. Molecular diversity based on Shannon’s diversity
indices (I) among 247 trees ranged from 0.268 to 0.350. In general,
the kelampayan trees in the three progeny trial blocks exhibited a high
level of molecular diversity and DNA polymorphism compared with its
natural populations. This preliminary information will form the base
for kelampayan tree improvement and conservation programmes. |
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