Staff View: Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium

Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium

Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastroin- testinal microbiota of humans and animals that confe...

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Main Authors:	Anis Syahirah, Saifor Adzuan, Sharifah Aminah, Syed Mohamad, Rashidah, Iberahim, Noor Nadia Syahira, Mohd Kamal, Nurliana, Abd Mutalib, Nur Intan, Hasbullah, Muneer, Alsaydi, Nor'aishah, Hasan, Low, Kheng Oon, Olaide, Olawunmi Ajibola, Rozila, Alias, Maimunah, Mustakim, Azlin, Sham Rambely, Emida, Mohamed, Mohammad Reza, Pourmand
Format:	Article
Language:	English
Published:	Tehran University of Medical Sciences. 2024
Subjects:	QR Microbiology S Agriculture (General)
Online Access:	http://ir.unimas.my/id/eprint/47312/1/IJM-16-763.pdf http://ir.unimas.my/id/eprint/47312/ https://ijm.tums.ac.ir/index.php/ijm/article/view/4746 https://doi.org/10.18502/ijm.v16i6.17254
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spelling	my.unimas.ir-473122025-01-08T07:31:07Z http://ir.unimas.my/id/eprint/47312/ Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium Anis Syahirah, Saifor Adzuan Sharifah Aminah, Syed Mohamad Rashidah, Iberahim Noor Nadia Syahira, Mohd Kamal Nurliana, Abd Mutalib Nur Intan, Hasbullah Muneer, Alsaydi Nor'aishah, Hasan Low, Kheng Oon Olaide, Olawunmi Ajibola Rozila, Alias Maimunah, Mustakim Azlin, Sham Rambely Emida, Mohamed Mohammad Reza, Pourmand QR Microbiology S Agriculture (General) Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastroin- testinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells. Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction. Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-iso- late from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Ty- phimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively. Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bacteri- cidal effects that serve to protect the host from infection. Tehran University of Medical Sciences. 2024-12 Article PeerReviewed text en http://ir.unimas.my/id/eprint/47312/1/IJM-16-763.pdf Anis Syahirah, Saifor Adzuan and Sharifah Aminah, Syed Mohamad and Rashidah, Iberahim and Noor Nadia Syahira, Mohd Kamal and Nurliana, Abd Mutalib and Nur Intan, Hasbullah and Muneer, Alsaydi and Nor'aishah, Hasan and Low, Kheng Oon and Olaide, Olawunmi Ajibola and Rozila, Alias and Maimunah, Mustakim and Azlin, Sham Rambely and Emida, Mohamed and Mohammad Reza, Pourmand (2024) Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium. Iranian Journal of Microbiology, 16 (6). pp. 763-772. ISSN 2008-3289 https://ijm.tums.ac.ir/index.php/ijm/article/view/4746 https://doi.org/10.18502/ijm.v16i6.17254
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	QR Microbiology S Agriculture (General)
spellingShingle	QR Microbiology S Agriculture (General) Anis Syahirah, Saifor Adzuan Sharifah Aminah, Syed Mohamad Rashidah, Iberahim Noor Nadia Syahira, Mohd Kamal Nurliana, Abd Mutalib Nur Intan, Hasbullah Muneer, Alsaydi Nor'aishah, Hasan Low, Kheng Oon Olaide, Olawunmi Ajibola Rozila, Alias Maimunah, Mustakim Azlin, Sham Rambely Emida, Mohamed Mohammad Reza, Pourmand Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
description	Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastroin- testinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells. Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction. Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-iso- late from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Ty- phimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively. Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bacteri- cidal effects that serve to protect the host from infection.
format	Article
author	Anis Syahirah, Saifor Adzuan Sharifah Aminah, Syed Mohamad Rashidah, Iberahim Noor Nadia Syahira, Mohd Kamal Nurliana, Abd Mutalib Nur Intan, Hasbullah Muneer, Alsaydi Nor'aishah, Hasan Low, Kheng Oon Olaide, Olawunmi Ajibola Rozila, Alias Maimunah, Mustakim Azlin, Sham Rambely Emida, Mohamed Mohammad Reza, Pourmand
author_facet	Anis Syahirah, Saifor Adzuan Sharifah Aminah, Syed Mohamad Rashidah, Iberahim Noor Nadia Syahira, Mohd Kamal Nurliana, Abd Mutalib Nur Intan, Hasbullah Muneer, Alsaydi Nor'aishah, Hasan Low, Kheng Oon Olaide, Olawunmi Ajibola Rozila, Alias Maimunah, Mustakim Azlin, Sham Rambely Emida, Mohamed Mohammad Reza, Pourmand
author_sort	Anis Syahirah, Saifor Adzuan
title	Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
title_short	Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
title_full	Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
title_fullStr	Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
title_full_unstemmed	Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
title_sort	anti-invasion activities of heat-killed lactic acid bacteria isolates against salmonella enterica serovar typhimurium
publisher	Tehran University of Medical Sciences.
publishDate	2024
url	http://ir.unimas.my/id/eprint/47312/1/IJM-16-763.pdf http://ir.unimas.my/id/eprint/47312/ https://ijm.tums.ac.ir/index.php/ijm/article/view/4746 https://doi.org/10.18502/ijm.v16i6.17254
_version_	1821007935940067328
score	13.244413

Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium

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