Cover Image

Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring

This study focused on the isolation and identification of the thermostable bacteria that produce the most highly enzyme activity obtained from Anah Rais Hot Spring. Four selective media have been used namely xylan, starch, CarboxymethyIcellulase (CMC) and skim milk media to isolate specific ba...

Full description

Saved in:
Bibliographic Details
Main Author: Mohd Fuad Amir Ruzal, .
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2006
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/46675/1/Mohd%20Fuad%20ft.pdf
http://ir.unimas.my/id/eprint/46675/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir-46675
record_format eprints
spelling my.unimas.ir-466752024-11-20T08:07:52Z http://ir.unimas.my/id/eprint/46675/ Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring Mohd Fuad Amir Ruzal, . Q Science (General) This study focused on the isolation and identification of the thermostable bacteria that produce the most highly enzyme activity obtained from Anah Rais Hot Spring. Four selective media have been used namely xylan, starch, CarboxymethyIcellulase (CMC) and skim milk media to isolate specific bacteria from the water sample. The growth of the culture been observed and comparison was done among those different media to select the one that produce the most highly enzyme activity. The enzyme activity was indicated by enzyme degradation of the each media that produced clear haloes around the supernatant (containing the enzyme) of each microorganism. Among those four, the growth of the skim milk media was selected to been studied further onto it morphological and molecular level since it produced the biggest size of halo. Throughout the study, 5 colonies of the proteolytic organisms have been isolated and identified as Sm i Nisseria sp., Sm2 Bacillus sp., Sm3 Lnterohacter sp., Sm4 Klehsiella sp. and Sm5 Streptococcus sp. Biochemical test was carried out in order to identify and differentiate the species. Genomic DNA from each bacteria colony was extracted. However, no further work was carried out due to time limitation. Universiti Malaysia Sarawak, (UNIMAS) 2006 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/46675/1/Mohd%20Fuad%20ft.pdf Mohd Fuad Amir Ruzal, . (2006) Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Mohd Fuad Amir Ruzal, .
Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
description This study focused on the isolation and identification of the thermostable bacteria that produce the most highly enzyme activity obtained from Anah Rais Hot Spring. Four selective media have been used namely xylan, starch, CarboxymethyIcellulase (CMC) and skim milk media to isolate specific bacteria from the water sample. The growth of the culture been observed and comparison was done among those different media to select the one that produce the most highly enzyme activity. The enzyme activity was indicated by enzyme degradation of the each media that produced clear haloes around the supernatant (containing the enzyme) of each microorganism. Among those four, the growth of the skim milk media was selected to been studied further onto it morphological and molecular level since it produced the biggest size of halo. Throughout the study, 5 colonies of the proteolytic organisms have been isolated and identified as Sm i Nisseria sp., Sm2 Bacillus sp., Sm3 Lnterohacter sp., Sm4 Klehsiella sp. and Sm5 Streptococcus sp. Biochemical test was carried out in order to identify and differentiate the species. Genomic DNA from each bacteria colony was extracted. However, no further work was carried out due to time limitation.
format Final Year Project Report
author Mohd Fuad Amir Ruzal, .
author_facet Mohd Fuad Amir Ruzal, .
author_sort Mohd Fuad Amir Ruzal, .
title Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
title_short Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
title_full Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
title_fullStr Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
title_full_unstemmed Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
title_sort diversity of thermophilic bacteria isolated from anah rais hot spring
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/46675/1/Mohd%20Fuad%20ft.pdf
http://ir.unimas.my/id/eprint/46675/
_version_ 1817848752792141824
score 13.223943

Similar Items