Diversity of Thermophilic Bacteria Isolated From Anah Rais Hot Spring
This study focused on the isolation and identification of the thermostable bacteria that produce the most highly enzyme activity obtained from Anah Rais Hot Spring. Four selective media have been used namely xylan, starch, CarboxymethyIcellulase (CMC) and skim milk media to isolate specific ba...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2006
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/46675/1/Mohd%20Fuad%20ft.pdf http://ir.unimas.my/id/eprint/46675/ |
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| Summary: | This study focused on the isolation and identification of the thermostable bacteria that
produce the most highly enzyme activity obtained from Anah Rais Hot Spring. Four selective
media have been used namely xylan, starch, CarboxymethyIcellulase (CMC) and skim milk
media to isolate specific bacteria from the water sample. The growth of the culture been
observed and comparison was done among those different media to select the one that
produce the most highly enzyme activity. The enzyme activity was indicated by enzyme
degradation of the each media that produced clear haloes around the supernatant (containing
the enzyme) of each microorganism. Among those four, the growth of the skim milk media
was selected to been studied further onto it morphological and molecular level since it
produced the biggest size of halo. Throughout the study, 5 colonies of the proteolytic
organisms have been isolated and identified as Sm i Nisseria sp., Sm2 Bacillus sp., Sm3
Lnterohacter sp., Sm4 Klehsiella sp. and Sm5 Streptococcus sp. Biochemical test was carried
out in order to identify and differentiate the species. Genomic DNA from each bacteria
colony was extracted. However, no further work was carried out due to time limitation. |
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