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Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography

Biogenic amines (BAs) are organic basis formed in food by microorganisms through enzymatic decarboxylation of amino acid, non volatile and heat stable. Biogenic amine can be found in several of food especially food that inappropriate handle and stored scombroid fish like tuna. The aim of this study...

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Main Author: Nur Idatul Diana, bt Ahmad Zaki
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/10410/5/Nur%20Idatul%20Diana%20ft.pdf
http://ir.unimas.my/id/eprint/10410/
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spelling my.unimas.ir-104102025-02-21T07:25:25Z http://ir.unimas.my/id/eprint/10410/ Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography Nur Idatul Diana, bt Ahmad Zaki SH Aquaculture. Fisheries. Angling Biogenic amines (BAs) are organic basis formed in food by microorganisms through enzymatic decarboxylation of amino acid, non volatile and heat stable. Biogenic amine can be found in several of food especially food that inappropriate handle and stored scombroid fish like tuna. The aim of this study was for the verification of an high performance liquid chromatography (HPLC) method for the determination of biogenic amines in fish. The implementation of the method included the optimization of sample preparation, HPLC analysis and data evaluation. Thereafter, the method performance was verified to demonstrate that the results obtained were consistent, correct and satisfactory for analysis of biogenic amines in fish. Separation of biogenic amine was achieved on a Purospher® STAR column with gradient elution system coupled with a UV detector (198 nm). A mixture of standard biogenic amines consist of tyramine, cadaverine and histamine were used for the verification test such as linearity, limit of detection, limit of quantification, accuracy, precison and recovery. The calibration curves for biogenic amines are linear from the concentration of 10 ppm until 75 ppm and linearity coefficients (r2) >0.900 for tyramine and histamine. Relative standard deviation (RSD) for biogenic amines <10% which are 4.74%, 5.15% and 5.53% for tyramine, cadaverine and histamine. The recovery in the concentration of tyramine and cadaverine are 86% and 95%, respectively. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10410/5/Nur%20Idatul%20Diana%20ft.pdf Nur Idatul Diana, bt Ahmad Zaki (2015) Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography. [Final Year Project Report]
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Nur Idatul Diana, bt Ahmad Zaki
Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
description Biogenic amines (BAs) are organic basis formed in food by microorganisms through enzymatic decarboxylation of amino acid, non volatile and heat stable. Biogenic amine can be found in several of food especially food that inappropriate handle and stored scombroid fish like tuna. The aim of this study was for the verification of an high performance liquid chromatography (HPLC) method for the determination of biogenic amines in fish. The implementation of the method included the optimization of sample preparation, HPLC analysis and data evaluation. Thereafter, the method performance was verified to demonstrate that the results obtained were consistent, correct and satisfactory for analysis of biogenic amines in fish. Separation of biogenic amine was achieved on a Purospher® STAR column with gradient elution system coupled with a UV detector (198 nm). A mixture of standard biogenic amines consist of tyramine, cadaverine and histamine were used for the verification test such as linearity, limit of detection, limit of quantification, accuracy, precison and recovery. The calibration curves for biogenic amines are linear from the concentration of 10 ppm until 75 ppm and linearity coefficients (r2) >0.900 for tyramine and histamine. Relative standard deviation (RSD) for biogenic amines <10% which are 4.74%, 5.15% and 5.53% for tyramine, cadaverine and histamine. The recovery in the concentration of tyramine and cadaverine are 86% and 95%, respectively.
format Final Year Project Report
author Nur Idatul Diana, bt Ahmad Zaki
author_facet Nur Idatul Diana, bt Ahmad Zaki
author_sort Nur Idatul Diana, bt Ahmad Zaki
title Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
title_short Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
title_full Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
title_fullStr Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
title_full_unstemmed Analysis of Biogenic Amines in fish by using High Performance Liquid Chromatography
title_sort analysis of biogenic amines in fish by using high performance liquid chromatography
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10410/5/Nur%20Idatul%20Diana%20ft.pdf
http://ir.unimas.my/id/eprint/10410/
_version_ 1825166825813442560
score 13.244413

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