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Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck

The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been st...

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Main Author: Nor Alina, Nazri
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2015
Subjects:
Ascorbic acid
Citrus grandis
Phosphate
Nitrate
Sucrose
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/40717
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spelling my.unimap-407172015-12-31T13:34:47Z Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck Nor Alina, Nazri Ascorbic acid Citrus grandis Phosphate Nitrate Sucrose The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been studied on their influence in production of ascorbic acid in C. grandis suspension cultures. The result obtained in factorial design indicates that among all four factors, only sucrose and incubation time give significant effect when present alone. However, all four factors have influence in the production when present together. This result was verified by the significant interaction among all the four factors. Then, suitable ranges of concentration for selected factors that obtained from factorial design were used in the optimization study. By using response surface methodology (RSM) through central composite design (CCD), the optimum conditions of selected factors were obtained. The result shows that when callus was supplied with 506.3mg/l phosphate, 1916.7 mg/l nitrate and 65.2 g/l sucrose and incubated in dark room for 7 days, maximum production of ascorbic acid at 34.53 ± 2.20 mg/l was obtained. The results obtained were in the range of predicted ascorbic acid and therefore the model of the study is acceptable. Then, the cultivation of callus in a bioreactor was carried out. The concentration of ascorbic acid obtained was 9 mg/l of which is very low compared to the production obtained in flask. This is due to the restriction provided by the unsuitable condition in the bioreactor for the growth of callus. 2015-12-31T13:34:47Z 2015-12-31T13:34:47Z 2010 Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/40717 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Ascorbic acid
Citrus grandis
Phosphate
Nitrate
Sucrose
spellingShingle Ascorbic acid
Citrus grandis
Phosphate
Nitrate
Sucrose
Nor Alina, Nazri
Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
description The potential of C. grandis tissue in producing high amount of ascorbic acid has been investigated in this study. In tissue culture technique, nutrients in the media have been a major factor in manipulating the final yield required. Phosphate, nitrate and sucrose with incubation time have been studied on their influence in production of ascorbic acid in C. grandis suspension cultures. The result obtained in factorial design indicates that among all four factors, only sucrose and incubation time give significant effect when present alone. However, all four factors have influence in the production when present together. This result was verified by the significant interaction among all the four factors. Then, suitable ranges of concentration for selected factors that obtained from factorial design were used in the optimization study. By using response surface methodology (RSM) through central composite design (CCD), the optimum conditions of selected factors were obtained. The result shows that when callus was supplied with 506.3mg/l phosphate, 1916.7 mg/l nitrate and 65.2 g/l sucrose and incubated in dark room for 7 days, maximum production of ascorbic acid at 34.53 ± 2.20 mg/l was obtained. The results obtained were in the range of predicted ascorbic acid and therefore the model of the study is acceptable. Then, the cultivation of callus in a bioreactor was carried out. The concentration of ascorbic acid obtained was 9 mg/l of which is very low compared to the production obtained in flask. This is due to the restriction provided by the unsuitable condition in the bioreactor for the growth of callus.
format Thesis
author Nor Alina, Nazri
author_facet Nor Alina, Nazri
author_sort Nor Alina, Nazri
title Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
title_short Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
title_full Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
title_fullStr Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
title_full_unstemmed Optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of Citrus Grandis (L.) Osbeck
title_sort optimization of phosphate, nitrate, sucrose and incubation time on the production of ascorbic acid in suspended callus of citrus grandis (l.) osbeck
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2015
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/40717
_version_ 1643799415538843648
score 13.211869

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