Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
Ulam are important food among the Malays because of their nutritional values, and their consumption is increasing. However, the foodborne outbreak which is associated with eating raw ulam may pose health risks to the consumers by increasing crosscontamination, antibiotic-resistant bacteria and...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Universiti Malaysia Terengganu
2022
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| Subjects: | |
| Online Access: | http://umt-ir.umt.edu.my:8080/handle/123456789/16525 |
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| Summary: | Ulam are important food among the Malays because of their nutritional values, and
their consumption is increasing. However, the foodborne outbreak which is associated
with eating raw ulam may pose health risks to the consumers by increasing crosscontamination,
antibiotic-resistant
bacteria
and
biofilm
formation.
The
objectives
of
this
study were to determine the phenotypic characterisation and genotypic
identification of E. coli and Salmonella isolated from ulam and their association with
biofilm. A total of 32 samples of ulam were randomly collected from wet markets and
supermarkets in Kuala Terengganu. The microbiological study was done to enumerate
microbial counts and to isolate E. coli and Salmonella using biochemical tests and
API20E kit. They were further assessed for phenotypic characteristics such as
antibiotic susceptibility testing, colony morphology and biofilm formation.
Polymerase chain reaction (PCR) was performed to determine the genotypic
identification associated with tetracycline resistance and biofilm formation in E. coli
and Salmonella isolates. The microbiological quality of ulam samples were in the
range of 6.36-8.83; 4.14-7.48; 0-8.16; 3.94-6.45 log10 CFU/g for aerobic mesophilic
bacteria, coliforms, Salmonella and yeast and moulds, respectively. E. coli and
Salmonella were detected in 31.3% and 9.4% of ulam samples, respectively. All of the
selected isolates were confirmed as E. coli and Salmonella enterica based on the
molecular identification using the 16S rRNA and invA gene, respectively. Overall, it
was found that the E. coli isolates were highly resistant to cephalothin (78.3%),
followed by ampicillin (52.2%), tetracycline (52.2%) and streptomycin (30.4%). |
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