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Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat

Pneumonic mannheimiosis is a common respiratory disease among small ruminants caused by Mannheimia haemolytica A2. Presently, the most efficient and costeffective mean of protection against this disease is through proper intranasal vaccination but the commercially available vaccines shown poor im...

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Main Author: Insyirah husna binti kamaradin
Format: Thesis
Language:English
Published: Universiti Malaysia Terengganu 2022
Online Access:http://umt-ir.umt.edu.my:8080/handle/123456789/15595
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spelling my.umt.ir-155952022-01-17T05:01:01Z Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat Insyirah husna binti kamaradin Pneumonic mannheimiosis is a common respiratory disease among small ruminants caused by Mannheimia haemolytica A2. Presently, the most efficient and costeffective mean of protection against this disease is through proper intranasal vaccination but the commercially available vaccines shown poor immunogenicity responses, or low protection level. Therefore, improving the vaccine via stimulating greater immune response requires incorporation of adjuvant. This study measures the potential of exopolysaccharide (EPS) extracted from Chlorella vulgaris as an adjuvant vaccine that can trigger the humoral and cellular immune response in both mucosal and systemic immunity against M. haemolytica A2 infection. Initially, prior to formation of Exopolysaccharide- M. haemolytica A2 (EPS-MHA2) vaccine, tests conducted to verify EPS’s characteristics which include using of Gel Permeation Chromatography (GPC) shown its molecular weight of 2398 Da. Its typical signals for polysaccharides were detected by Fourier Transform Infrared Spectra (FTIR). Scanning Electron Microscope (SEM) displayed the compact microstructure of EPS surface view while cytotoxicity test proved EPS nontoxicity. Subsequently, the potentiality of EPS-MHA2 as adjuvanted vaccine was put to pre-clinical test on white rats to determine its ability to enhance and stimulate immune response, which indicated by the significant high-level production of antibody (IgM, IgG and IgA) in blood serum of Sprague Dawley. 2022-01-17T05:00:59Z 2022-01-17T05:00:59Z 2020 Thesis http://umt-ir.umt.edu.my:8080/handle/123456789/15595 en application/pdf application/pdf Universiti Malaysia Terengganu
institution Universiti Malaysia Terengganu
building Perpustakaan Sultanah Nur Zahirah
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Terengganu
content_source UMT-IR
url_provider http://umt-ir.umt.edu.my:8080/
language English
description Pneumonic mannheimiosis is a common respiratory disease among small ruminants caused by Mannheimia haemolytica A2. Presently, the most efficient and costeffective mean of protection against this disease is through proper intranasal vaccination but the commercially available vaccines shown poor immunogenicity responses, or low protection level. Therefore, improving the vaccine via stimulating greater immune response requires incorporation of adjuvant. This study measures the potential of exopolysaccharide (EPS) extracted from Chlorella vulgaris as an adjuvant vaccine that can trigger the humoral and cellular immune response in both mucosal and systemic immunity against M. haemolytica A2 infection. Initially, prior to formation of Exopolysaccharide- M. haemolytica A2 (EPS-MHA2) vaccine, tests conducted to verify EPS’s characteristics which include using of Gel Permeation Chromatography (GPC) shown its molecular weight of 2398 Da. Its typical signals for polysaccharides were detected by Fourier Transform Infrared Spectra (FTIR). Scanning Electron Microscope (SEM) displayed the compact microstructure of EPS surface view while cytotoxicity test proved EPS nontoxicity. Subsequently, the potentiality of EPS-MHA2 as adjuvanted vaccine was put to pre-clinical test on white rats to determine its ability to enhance and stimulate immune response, which indicated by the significant high-level production of antibody (IgM, IgG and IgA) in blood serum of Sprague Dawley.
format Thesis
author Insyirah husna binti kamaradin
spellingShingle Insyirah husna binti kamaradin
Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
author_facet Insyirah husna binti kamaradin
author_sort Insyirah husna binti kamaradin
title Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
title_short Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
title_full Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
title_fullStr Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
title_full_unstemmed Application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
title_sort application of exopolysaccharide produced by chlorella vulgaris as adjuvant for mannheimia haemolytica a2 vaccine in goat
publisher Universiti Malaysia Terengganu
publishDate 2022
url http://umt-ir.umt.edu.my:8080/handle/123456789/15595
_version_ 1738395565521108992
score 13.244413

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