Staff View: Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)

Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)

Trichoderma species are well-known biological control agents (BCAs) that have significant antagonistic activity against various fungal phytopathogens. On the other hand, Ganoderma boninense has been identified as the phytopathogen causing basal stem rot (BSR), a devastating disease in oil palm crop...

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Main Authors:	Jumiati Asis, Nur Aainaa Hasbullah, Mohamadu Boyie Jalloh, Noor Khairani Mohamad Basri, Palanivell Perumal, Peter Mojiun, Mohd. Rashid Mohd. Rakib
Format:	Article
Language:	English
Published:	Sarhad Journal of Agriculture 2024
Subjects:	QK1-989 Botany QK504-(638) Cryptogams
Online Access:	https://eprints.ums.edu.my/id/eprint/42344/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42344/ https://dx.doi.org/10.17582/journal.sja/2024/40/s1.173.185
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spelling	my.ums.eprints.423442024-12-23T02:52:28Z https://eprints.ums.edu.my/id/eprint/42344/ Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis) Jumiati Asis Nur Aainaa Hasbullah Mohamadu Boyie Jalloh Noor Khairani Mohamad Basri Palanivell Perumal Peter Mojiun Mohd. Rashid Mohd. Rakib QK1-989 Botany QK504-(638) Cryptogams Trichoderma species are well-known biological control agents (BCAs) that have significant antagonistic activity against various fungal phytopathogens. On the other hand, Ganoderma boninense has been identified as the phytopathogen causing basal stem rot (BSR), a devastating disease in oil palm crop (Elaeis guineensis). In this study, the in vitro and in planta inhibition of G. boninense using Trichoderma isolates were evaluated. A total of 20 Trichoderma isolates were collected. Among the isolates, T4RH and T8R were selected for further in planta experiments as they showed significant inhibitory activity against G. boninense via in vitro dual-culture and dual-plate assays. Isolates T4RH and T8R were identified as Trichoderma virens and Trichoderma asperellum by the internal transcribed spacer gene sequences. The in-planta experiment was conducted by inoculation of 3-month-old oil palm seedlings with rubber wood block inoculum. Single and mixture conidial suspensions 1×106 of T4RH and T8R were applied to the Ganoderma-inoculated plants. It was found that T4RH, T8R, and mixed Trichoderma treatments have significantly lower disease incidence, disease severity index, area under disease progress curve, and percentage of necrotic primary roots as compared to the positive control. The disease reduction was up to 57.70% and 43.86% when plants were treated with T4RH and T8R, respectively. Additionally, the Trichoderma treatment recorded significantly higher chlorophyll content, plant height, bole diameter, and number of fronds as compared to the positive control. The findings from this study suggested that T. virens T4RH and T. asperellum T8R have the potential to be used as promising BCAs against G. boninense. Sarhad Journal of Agriculture 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42344/1/FULL%20TEXT.pdf Jumiati Asis and Nur Aainaa Hasbullah and Mohamadu Boyie Jalloh and Noor Khairani Mohamad Basri and Palanivell Perumal and Peter Mojiun and Mohd. Rashid Mohd. Rakib (2024) Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis). Sarhad Journal of Agriculture. pp. 1-13. https://dx.doi.org/10.17582/journal.sja/2024/40/s1.173.185
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	QK1-989 Botany QK504-(638) Cryptogams
spellingShingle	QK1-989 Botany QK504-(638) Cryptogams Jumiati Asis Nur Aainaa Hasbullah Mohamadu Boyie Jalloh Noor Khairani Mohamad Basri Palanivell Perumal Peter Mojiun Mohd. Rashid Mohd. Rakib Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
description	Trichoderma species are well-known biological control agents (BCAs) that have significant antagonistic activity against various fungal phytopathogens. On the other hand, Ganoderma boninense has been identified as the phytopathogen causing basal stem rot (BSR), a devastating disease in oil palm crop (Elaeis guineensis). In this study, the in vitro and in planta inhibition of G. boninense using Trichoderma isolates were evaluated. A total of 20 Trichoderma isolates were collected. Among the isolates, T4RH and T8R were selected for further in planta experiments as they showed significant inhibitory activity against G. boninense via in vitro dual-culture and dual-plate assays. Isolates T4RH and T8R were identified as Trichoderma virens and Trichoderma asperellum by the internal transcribed spacer gene sequences. The in-planta experiment was conducted by inoculation of 3-month-old oil palm seedlings with rubber wood block inoculum. Single and mixture conidial suspensions 1×106 of T4RH and T8R were applied to the Ganoderma-inoculated plants. It was found that T4RH, T8R, and mixed Trichoderma treatments have significantly lower disease incidence, disease severity index, area under disease progress curve, and percentage of necrotic primary roots as compared to the positive control. The disease reduction was up to 57.70% and 43.86% when plants were treated with T4RH and T8R, respectively. Additionally, the Trichoderma treatment recorded significantly higher chlorophyll content, plant height, bole diameter, and number of fronds as compared to the positive control. The findings from this study suggested that T. virens T4RH and T. asperellum T8R have the potential to be used as promising BCAs against G. boninense.
format	Article
author	Jumiati Asis Nur Aainaa Hasbullah Mohamadu Boyie Jalloh Noor Khairani Mohamad Basri Palanivell Perumal Peter Mojiun Mohd. Rashid Mohd. Rakib
author_facet	Jumiati Asis Nur Aainaa Hasbullah Mohamadu Boyie Jalloh Noor Khairani Mohamad Basri Palanivell Perumal Peter Mojiun Mohd. Rashid Mohd. Rakib
author_sort	Jumiati Asis
title	Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
title_short	Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
title_full	Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
title_fullStr	Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
title_full_unstemmed	Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)
title_sort	trichoderma species’ in vitro and in planta inhibition against ganoderma boninense, a causative agent of basal stem rot in oil palm (elaeis guineensis)
publisher	Sarhad Journal of Agriculture
publishDate	2024
url	https://eprints.ums.edu.my/id/eprint/42344/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42344/ https://dx.doi.org/10.17582/journal.sja/2024/40/s1.173.185
_version_	1819911287741612032
score	13.223943

Trichoderma species’ in vitro and in planta inhibition against Ganoderma Boninense, a causative agent of basal stem rot in oil palm (Elaeis guineensis)

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