Cover Image

Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita

Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloi...

Full description

Saved in:
Bibliographic Details
Main Author: Fatin Ruzanna Jamaluddin
Format: Thesis
Language:English
English
Published: 2013
Subjects:
QK710-899 Plant physiology
Online Access:https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/41791/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.41791
record_format eprints
spelling my.ums.eprints.417912024-11-29T04:53:38Z https://eprints.ums.edu.my/id/eprint/41791/ Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita Fatin Ruzanna Jamaluddin QK710-899 Plant physiology Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloids, phytosterols, saponins and phenolic compounds were present in the sapwood, heartwood and bark, whereas only phytosterols were present in hexane extracts. Therefore, determination of antioxidant and antimicrobial activities were carried out only for the methanolic crude extracts of wood and bark. The methanolic crude extracts were tested for primary and secondary antioxidant properties by measuring the total phenolic contents (TPC) and the 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The extracts demonstrated higher TPCs and free radical scavenging activities against DPPH, especially for the bark and most parts of young wood as compared to matured wood of N. subdita . Study was also carried out to test antimicrobial activities of the methanolic crude extracts against four bacteria, i.e. Bacillus subtilis, Vibrio parahaemoliticus, Vibrio alginolyticus, Bukholderia cepacia, and two fungi, i.e. Candida albican and Aspergillus niger . The results indicated that all the samples have moderate to good zones of inhibition on the bacteria growth when compared to the positive control. However, there was no zone of inhibition against the two fungi (A. niger and C. albican ) used. Gas chromatography-mass spectrometry (GC-MS) technique was also employed for the analysis of semi-volatile compounds in six selected crude extracts from young and matured woods of N. subdita . The groups of compound were identified are from hydrocarbon, alcohol, ester, phenol, ether, ketone, organofluorine, organoclorine and organic acid groups. 2013 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf Fatin Ruzanna Jamaluddin (2013) Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QK710-899 Plant physiology
spellingShingle QK710-899 Plant physiology
Fatin Ruzanna Jamaluddin
Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
description Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloids, phytosterols, saponins and phenolic compounds were present in the sapwood, heartwood and bark, whereas only phytosterols were present in hexane extracts. Therefore, determination of antioxidant and antimicrobial activities were carried out only for the methanolic crude extracts of wood and bark. The methanolic crude extracts were tested for primary and secondary antioxidant properties by measuring the total phenolic contents (TPC) and the 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The extracts demonstrated higher TPCs and free radical scavenging activities against DPPH, especially for the bark and most parts of young wood as compared to matured wood of N. subdita . Study was also carried out to test antimicrobial activities of the methanolic crude extracts against four bacteria, i.e. Bacillus subtilis, Vibrio parahaemoliticus, Vibrio alginolyticus, Bukholderia cepacia, and two fungi, i.e. Candida albican and Aspergillus niger . The results indicated that all the samples have moderate to good zones of inhibition on the bacteria growth when compared to the positive control. However, there was no zone of inhibition against the two fungi (A. niger and C. albican ) used. Gas chromatography-mass spectrometry (GC-MS) technique was also employed for the analysis of semi-volatile compounds in six selected crude extracts from young and matured woods of N. subdita . The groups of compound were identified are from hydrocarbon, alcohol, ester, phenol, ether, ketone, organofluorine, organoclorine and organic acid groups.
format Thesis
author Fatin Ruzanna Jamaluddin
author_facet Fatin Ruzanna Jamaluddin
author_sort Fatin Ruzanna Jamaluddin
title Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_short Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_full Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_fullStr Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_full_unstemmed Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
title_sort antioxidant and antimicrobial properties of chemical extractives from nauclea subdita
publishDate 2013
url https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/41791/
_version_ 1817843844470800384
score 13.223943

Similar Items