Antioxidant and antimicrobial properties of chemical extractives from Nauclea subdita
Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloi...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/41791/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/41791/2/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/41791/ |
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| Summary: | Solvent extraction of young and matured woods and barks of Nauclea subdita in polar solvent (methanol) and non-polar solvent (hexane) have been carried out in this study. Phytochemical screening tests of the crude extracts of Nauclea subdita in methanol indicated that flavanoids, terpenoids, alkaloids, phytosterols, saponins and phenolic compounds were present in the sapwood, heartwood and bark, whereas only phytosterols were present in hexane extracts. Therefore, determination of antioxidant and antimicrobial activities were carried out only for the methanolic crude extracts of wood and bark. The methanolic crude extracts were tested for primary and secondary antioxidant properties by measuring the total phenolic contents (TPC) and the 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The extracts demonstrated higher TPCs and free radical scavenging activities against DPPH, especially for the bark and most parts of young wood as compared to matured wood of N. subdita . Study was also carried out to test antimicrobial activities of the methanolic crude extracts against four bacteria, i.e. Bacillus subtilis, Vibrio parahaemoliticus, Vibrio alginolyticus, Bukholderia cepacia, and two fungi, i.e. Candida albican and Aspergillus niger . The results indicated that all the samples have moderate to good zones of inhibition on the bacteria growth when compared to the positive control. However, there was no zone of inhibition against the two fungi (A. niger and C. albican ) used. Gas chromatography-mass spectrometry (GC-MS) technique was also employed for the analysis of semi-volatile compounds in six selected crude extracts from young and matured woods of N. subdita . The groups of compound were identified are from hydrocarbon, alcohol, ester, phenol, ether, ketone, organofluorine, organoclorine and organic acid groups. |
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